摘要
采用纯培养方法从新疆罗布泊盐湖中分离得到168株嗜盐细菌,采用核糖体DNA扩增片段限制性酶切分析(ARDRA)研究了罗布泊嗜盐细菌的群落结构和多样性。通过限制性内切酶HinfI对108个菌株的16SrDNA进行酶切分型,根据ARDRA的酶切图谱,将其划分为12个操作分类单元。16SrDNA序列测定和系统发育分析结果显示,分离菌株分布于喜盐芽孢杆菌属(Halobacillus)、芽孢杆菌属(Bacillus)、短杆菌属(Brevibacterium)、嗜盐单胞菌属(Halomonas)、色盐杆菌属(Chromohalobacter)、盐水球菌属(Salinicoccus)、库克菌属(Kocuria)、葡萄球菌属(Staphylococcus)、微球菌属(Micrococcus)等9个属及1个可能的新分类单元。其中Halomonas为优势菌群,Chromohalobacter、Halobacillus为次优势菌群。采用ERIC-PCR分析优势菌群Halomonas各菌株的基因组特征,显示出有21种指纹图谱。研究结果揭示,罗布泊嗜盐细菌不仅具有丰富的多样性,还蕴藏着具有地域特点的新菌种资源。
We investigated the community structure and diversity of the culturable halophilic bacteria in Lop Nur, Xinjiang using amplified ribosomal DNA restriction analysis （ARDRA）. Restriction enzyme Hinfl was applied in ARDRA of 108 halophilic bacterial strains, and 12 operational taxonomic units （OTUs） were assigned on the basis of the ARDRA patterns. Each OTU was randomly chosen for 16S rRNA gene sequencing. The phylogenetic analysis on the basis of 16S rRNA gene homology showed that all isolates affiliated to nine genera, consisting of Halobacillus, Bacillus, Brevibacterium, Halomonas, Chromohalobacter, Salinicoccus, Kocuria, Staphylococcus, Micrococcus, and a possible novel taxon. Halomonas strains dominanted among the isolates, and followed by Chromohalobacter and Halobacillus members. The strains of Italomonas were performed ERIC-PCR （enterobacterial repetitive intergenic consensus-PCR） analysis, and 21 ERIC-PCR fingerprinting types were determined. The results suggest that the halophilic bacteria are abundant, and some unknown taxa can exist in Lop Nur.
出处
《生物多样性》
CAS
CSCD
北大核心
2009年第3期288-295,共8页
Biodiversity Science
基金
国家自然科学基金(40671080)
新疆特殊环境微生物重点实验室开放项目(XJYS0203-2006-03)
