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抗坏血酸与过氧化氢反应的自旋捕捉┐ESR研究 被引量:9

The ESR Spin Trapping Studies of Reaction Between Ascorbic Acid with Hydrogen Peroxide
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摘要 利用自旋捕捉-ESR方法研究了分析纯抗坏血酸(Aa)与医用维生素C注射液(Vc)的自氧化和过氧化氢反应.在自氧化反应中只观察到去氢抗坏血酸负离子基A-·,而与过氧化氢反应时除观察到A-·外,还观测到另外数种自由基.经计算机拟合后得到这些自由基或其5,5-二甲基-1-吡咯啉-1-氧化物(DMPO)加合物的ESR谱参数,在此基础上推测出该反应的机理. Using 5,5 dimethyl 1 pyrroline N oxide(DMPO) as a spin trapping reagent, the oxidation reaction of ascorbic acid was studied. The radical generated in autoxidation process was ascorbate radical anion(A -\5 ) only. In the presence of hydrogen peroxide, several radical intermediates were found and their structure were determined. The ESR parameters of A -\5 and DMPO/radical adducts were measured to be g =2.005 6 and α H=0.17 mT for A -\5 , g =2.005 9, α N=1.60 mT and α β H=2.28 mT for DMPO/1, g =2.005 9, α N=1.58 mT, α β H=2.13 mT and α γ H=0.17 mT for DMPO/2, g =2.005 9 and α N= α β H=1.49 mT for DMPO/\5OH , and g =2.005 9 and α N=1.50 mT for DMPO/3(or 4). The mechanisms for the oxidation reactions of ascorbic acid were proposed on the basis of those observation.
机构地区 南开大学化学系
出处 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 1998年第6期890-894,共5页 Chemical Journal of Chinese Universities
关键词 抗坏血酸 自旋捕捉 ESR 过氧化氢 羟基自由基 Ascorbic acid, ESR spin trapping, Hydrogen peroxide, Ascorbate radical anion
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参考文献1

  • 1Zhao B L,Cell Biophys,1989年,14卷,175页

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