摘要
制备了β－环糊精交联聚合物（β－ＣＤＰ）和四（４－三甲铵基苯基）卟啉锰（ＭｎＴＡＰＰ）的超分子包合物（ＭｎＴＡＰＰ－β－ＣＤＰ），并以此包合物作为过氧化物朊酶的模型物．结果表明，固载化的包合物不仅十分稳定，而且呈现出较高的过氧化物朊酶活性．将它应用于酶法分析，可催化过氧化氢与４－氨基安替比林和对氯苯酚的显色反应，当过氧化氢浓度为０～２．５μｇ／ｍＬ时，与吸光度呈良好线性关系，相关系数为０．９９９２．
The supramolecular inclusive complex of 5,10,15,20-tetrakis[4-(trimethylammonium)pheny1]-21H,23H-porphine manganese (Ⅱ)(MnTAPP)with β-cyclodextrin cross-linking polymer (β-CDP) was obtained and used as an analogue for peroxide proteinase. The immobilized inclusive complex as a mimetic enzyme of horseradish peroxidase was very stable exhibited the high proteinase-like activity, which has been demonstrated by enzymatic methods of analysis. The method was applied to the determination of hydrogen peroxide with 4-aminoantipyrine and p-chlorophenic acid. The calibration graph for the determination of hydrogen peroxide was linear from 0 to 2.5μg/mL and the linear correlation coefficient was found as 0.9992.
出处
《分析科学学报》
CAS
CSCD
1998年第2期94-97,共4页
Journal of Analytical Science
基金
国家自然科学基金
关键词
过氧化氢
测定
分析
模型物
模拟
过氧化物朊酶
β-cyclodextrin cross-linking polymer, Mimetic peroxide proteinase, Metalloporphyrin, H_2O_2 determination
