摘要
目的:探讨不同糖耐量阶段血浆同型半胱氨酸(Hcy)与血清C反应蛋白(CRP)的改变及两者的关系。方法:测定新发糖尿病组(T2DM,30例),糖耐量低减组(IGT,35例),对照组即正常糖耐量组(NGT,40例)血浆Hcy、叶酸、VitB12和血清CRP的水平。Hcy采用免疫荧光偏振法测定,CRP采用酶联免疫法测定,化学发光法测定血浆叶酸、VitB12浓度。结果:NGT、IGT、DM各组中,血清CRP水平逐渐升高(P<0.001),IGT与新发DM组血浆Hcy显著高于NGT组(P<0.01),新发DM组Hcy略高于IGT组,但无统计学差异(P>0.05);叶酸、VitB12水平在NGT、IGT、DM各组中依次减低,且NGT组与IGT、DM组相比,差异有显著性(P<0.05)。相关分析显示Hcy与CRP(r=0.231,P=0.034)、尿Alb/Cr(r=0.327,P=0.001)、FBG(r=0.236,P=0.017)呈显著正相关关系,与叶酸(r=-0.294,P=0.003)、VitB12(r=-0.328,P=0.001)显著负相关。结论:随着糖耐量的恶化,血浆Hcy与血清CRP水平逐渐升高,可能与叶酸、VitB12的缺乏及胰岛素抵抗的加重有关。血浆Hcy与血清CRP可能参与了糖耐量异常患者血管病变的发生。
Objective： To investigate the changes of plasma homocysteine （Hey） and serum C reactive protein （CRP） levels in subjects with different glucose tolerance and their relation. Methods： A total of 105 subjects were recruited in this study. Among these subjects, 40 were healthy controls with normal glucose tolerance （ NGT）, 35 had impaired glucose tolerance （IGT）, 30 had newly diagnosed type 2 diabetes mellitus （DM）. Plasma Hey levels were measured by polarization immunoassay （FPIA） , serum CRP concentrations were determined by enzymatic linked immunosorbbent assay （ELISA） , plasma folic acid and vitamin B12 levels were measured by radioimmunoassay. Results： Plasma Hey concentrations were significantly higher in patients with IGT and DM than in patients with NGT [ median （25% and 75% ）, IGT 7.08（6.45 - 8.58） μmol/L, DM 8.58 （6.97 -9.46）μmol/L, NGT 5.64 （5.17 -8.00）μmol/L, P 〈0.05） ]. The serum CRP level [median （25%, 75% ） ] was 0.37 （0.18 - 0.89 ） mg/L in patients with NGT and 1.34 （ 0.53 - 2.79 ） mg/L in patients with IGT, 1.66 （ 1.08 - 2.27 ） mg/L in patients with type 2 DM （P 〈 0.05 ） respectively, while folie acid and vitamin B12 levels decreased gradually with the worsening glucose tolerance （ P 〈 0.05 ）. Plasma Hey concentrations were positively correlated with serum CRP and urine Alb/Cr levels （ r = 0.231, P = 0. 034 ; r = 0.327, P = 0. 001, respectively）, and negatively correlated with plasma folio acid and vitamin B12 levels （ r = - 0.294, P = 0. 003 ; r = - 0. 328, P = 0.001 ）. Conclusion： With the worsening of glucose tolerance, the Hey and CRP levels increase gradually. Deficiency in folic acid and vitamin B12 and insulin resistance might be responsible for the increased Hey and CRP levels in patients with abnormal glucose tolerance. Hey and CRP might partially account for the increased risk of developing vasculopathy in patients with abnormal glucose tolerance.
出处
《军医进修学院学报》
CAS
2009年第3期264-266,共3页
Academic Journal of Pla Postgraduate Medical School
基金
北京市首都医学科研发展基金联合攻关课题(2003-1009)~~
关键词
同型半胱氨酸
C反应蛋白质
糖耐量低减
糖尿病
homoeysteine
C-reactive protein
impaired glucose tolerance
diabetes mellitus
