摘要
利用RAPD技术对3个不同盐度梯度下入侵杂草一年蓬的遗传多样性和遗传分化进行了研究。结果表明,12个随机引物在90株个体中共检测到187个可重复位点,其中多态位点158个,总多态位点百分率为84.49%,3个种群平均多态位点百分率为70.77%。采用Shannon信息指数计算的3个种群总的遗传多样性为0.4522,平均为0.4027;采用Nei指数计算的3个种群总的基因多样性为0.3042,平均为0.2746。3个种群的多态位点百分率、Shannon信息指数、Nei指数大小顺序均为P2>P1>P3。AMOVA分子变异显示,88.54%变异来源于种群内,11.46%变异来源于种群间。一年蓬高的遗传多样性与低的遗传分化与其生物学特性有关,是其广泛入侵的重要原因。
Genetic diversity and differentiation of Erigeron annuns in three different salinity gradients were analyzed using random amplified polymorphic DNA （RAPD） technique. The results showed that 187 repetitive loci were produced in 90 individuals using 12 random primers, among which 158 loci were polyrnorphic and the total percentage of polymorphic loci were 84.49 % with an average of 70.77 %. Estimated by Shannon informative index, the genetic diversity was 0. 4522 with an average of 0. 4027. Estimated by NEI index, the total gene diversity was 0. 3042 with an average of 0. 2746. The percentage of polymorphic loci, Shannon informative index and Nei index all ranked in the decreased order：P2 〉 P1 〉 P3. Analysis of molecular variance showed that 88.54 % of variance existed within populations while 11.46 % of variance existed among populations. High genetic diversity and low genetic differentiation might be due to the biological characteristics of Erigeron annuns, which might be the main reason for the wide invasion.
出处
《福建林业科技》
2009年第2期44-47,92,共5页
Journal of Fujian Forestry Science and Technology
基金
浙江省自然科学基金项目(Y505331)
关键词
一年蓬
盐度梯度
RAPD
遗传多样性
遗传分化
Erigeron annuns
salinity gradient
RAPD
genetic diversity
genetic differentiation
