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苍术属植物的遗传关系研究 被引量:10

Genetic relationships of Atractylodes plants
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摘要 运用对PCR产物直接测序的方法测定了苍术属8种植物的ITS序列和叶绿体atpB-rbcL、psbB-psbF和trnL-trnF基因间隔区序列。计算了不同种间ITS序列、atpB-rbcL+psbB-psbF+trnL-trnF序列的碱基差别(转换值/颠换值)和Kimura遗传距离。序列数据经排列后进行UPGMA法分析,结果显示:①罗田苍术-苍术和辽东苍术-朝鲜苍术各自构成一个分支,再参照分支内各植物之间的遗传距离值,认为将罗田苍术作为苍术变种不适宜;②鄂西苍术与属内其余植物亲缘关系都较远,在属内处于原始地位;③支持将辽东苍术归入朝鲜苍术,将北苍术作为苍术的一个变种处理。 The phylogenetic relationships of the genus Atractylodes DC. was analyzed using the nuclear ribosomal ITS and three chloroplast fragments, including atpB-rbcL, psbB-psbF and trnL-trnF intergenic spacer (IGS) sequences. Phylogenetic analyses revealed that A. lancea subsp, luotianensis and A. lancea, A. chinensis var. liaotungensis and A. coreana form monophyletic terminal clade, separately. The trees, within each the pairwise genetic distances, did not support Hu's classification. Authors suggested that A. lancea Subsp. luotianensis should be included in A. lancea rather than be treated as a separate subspecies. A. carlinoides was placed in the basal position of Atractylodes, which had a distant relationship with the others of the genus. The results lead us to suggest that A. chinensis var. liaotungensis be put into A. coreana, A. chinensis as a subspecies ofA. lancea.
出处 《药学学报》 CAS CSCD 北大核心 2009年第6期680-686,共7页 Acta Pharmaceutica Sinica
基金 国家重点基础研究发展计划(973计划)资助项目(2006CB504700)
关键词 苍术属 ITS序列 ATPB-RBCL psbB-psbF TRNL-TRNF 遗传关系 Atractylodes DC. ITS sequence atpB-rbcL psbB-psbF trnL-trnF genetic relationship
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