摘要
生测结果表明，苦皮藤毒杀成分苦皮藤素Ⅴ若被试虫摄入消化道（胃毒处理），则在小地老虎Ａｇｒｏｔｉｓｙｐｓｉｌｏｎ和粘虫Ｍｙｔｈｉｍｎａｓｅｐａｒａｔａ幼虫之间有明显的选择毒杀作用：对小地老虎４龄幼虫，即使给予高达１０５ｍｇ／ｇ的剂量，试虫也不表现任何中毒症状，而对粘虫４龄幼虫，仅给予３７６μｇ／ｇ的剂量，试虫就表现失水、死亡。若直接将苦皮藤素Ⅴ注入试虫血淋巴，则小地老虎和粘虫４龄幼虫都表现相同的失水、死亡症状，不表现选择毒杀作用。这种胃毒选择毒杀作用的机理包括２个方面：①药剂在虫体内穿透作用的差异。苦皮藤素Ⅴ很容易从粘虫幼虫消化道穿透肠壁进入血腔，也容易从血腔进入消化道；但苦皮藤素Ⅴ既不能从小地老虎幼虫消化道进入血腔，也不能从血腔进入消化道；②试虫解毒能力的差异。活体试验和离体试验都说明小地老虎幼虫对苦皮藤素Ⅴ的解毒速率远大于粘虫幼虫。
The bioassay results of the poisonous ingredient celangulin Ⅴ against the 4thinstar larvae of Mythimna separata and Agrotis ypsilon with oral applicating method showed that celangulin Ⅴ had selective poisonous action:the larvae of Mythimna separata could lose the bodyliguid and fall into death at a dosage of 376 μg/g, no individual of the larva of Agrotis ypsilon would be exhibited any poisonous symptom, even though at a dosage of 105 mg/g The bioassay results of celangulin Ⅴ agaist the 4thinstar larvae of Mythimna separata and Agrotis ypsilon with injection applicating method showed that celangulin Ⅴ had no selective poisonous action, both Mythimna separata and Agrotis ypsilon larvae could lose the bodyliquid and fall into deathTwo respects of the mechanism might be involved in this selective toxicity:1The difference of penetrationIn the larval body of Mythimna separata, celangulin Ⅴ could penetrate the midgut wall from the digestive tube into the haemocoele, or from haemocoel into the digestive tube, while in the larval body of Agrotis ypsilon, celangulin Ⅴ could not penetrate the midgut wall neither from the digestive tube into the haemocoele, nor from the hacmocoele into the digestive tube2The difference of detoxicityThe testing results in Vivo or in Vitro indicated that the rate of degradation and detoxicity of celangulin Ⅴ in the larval body of Agrotis ypsilon was higher than in the larval bdy ofMythimna separata
出处
《西北农业学报》
CAS
CSCD
1998年第2期41-44,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金
