摘要
[目的]为掌握云南野生大花红景天的组织培养与快速繁殖技术。[方法]将叶片接种于培养基MS+BA 2 mg/L+IAA 0.2 mg/L,花芽接种在培养基MS+KT 2 mg/L上,茎尖接种在培养基MS+BA 2 mg/L+IAA 1 mg/L上,幼茎接种在培养基MS+KT 2 mg/L+IAA 2mg/L上,在培养条件下进行培养。[结果]以叶片为外植体诱导芽效果最好,分化率达100%;幼茎、茎尖与花芽分化时间长,且分化率较低,不宜作为快繁技术的外植体;在培养基MS+NAA 0.2 mg/L上,生根率达100%;通过炼苗后,成活率达70%左右。[结论]为大花红景天的快速繁殖提供了理论依据。
[ Objective] The study aimed to research tissue euhure and rapid propagation technique of Yunnan wild Rhodiola creanlata. [ Method] Inoculating leaves with culture medium MS ＋ BA 2 mg/L ＋ IAA 0.2 mg,/L, inoculating blossom bud with culture medium MS ＋ KT 2 mg/L, inoculating stem point with euhure medium MS ＋ BA 2 mg/L ＋ IAA 1 mg/L, inoculating immature stem with culture medium MS ＋ KT 2 mg/L ＋ IAA 2 mg/L, cultivated them under the condition. [Result] The effect was best using leaves as explants induce stem, induction percentages reached 100% ; the induction of immature stem, stem point, blossom bud not only took more time, and the percentages also teo low, they were not suit to take as explants for rapid propagation; the shoot haduetion percentage of culture medium MS ＋ NAA 0.2 mg/L reached 100% ; after having been hardened the regeneration plates ceuld survive at 70%. [ Conehtsion] Providing basis for rapid Propagation of Rhodiola crenulata.
出处
《安徽农业科学》
CAS
北大核心
2009年第17期7865-7865,7867,共2页
Journal of Anhui Agricultural Sciences
基金
云南省自然科学基金项目(2008ZC102M)
关键词
大花红景天
组织培养
快速繁殖
Rhodiola crenulata
Tissue culture
Raoid propagation
