摘要
目的:研究大鼠心肌缺血再灌注损伤心肌凋亡以及氯化锂的干预作用。方法:健康SD大鼠随机分4组:①正常对照组(A组,5只),②心肌缺血再灌注组(B组,15只),③心肌缺血再灌注+0.9%氯化钠组(C组,15只),④心肌缺血再灌注+氯化锂组(D组,15只),B,C,D组又分为1h、3h、6h3个亚组,每个亚组5只大鼠。流式细胞仪检测心肌细胞凋亡率,RT-PCR和WesternBlot分别检测凋亡相关基因蛋白Bcl-2和Caspase-3的表达。结果:与A组比较,B、C组3h心肌细胞凋亡率增高、Bcl-2减少、Casepase-3增多,差异有统计学意义;D组心肌细胞凋亡率下降、Bcl-2和Casepase-3表达与A组差异无统计学意义。结论:心肌再灌注损伤引起心肌细胞凋亡增多,Bcl-2降低以及Caspase-3增高,氯化锂能够减轻以上变化。
Objective： To study the effects of lithium chloride on apoptosis and the related proteins of myocardial cells in the rats with myocardial isehemia reperfusion injury. Method： SD rats were divided randomly into 4 groups： （1）normal control group（group A, n=5）, （2）myocardial ischemia reperfusion （MIR） group（group B, n= 15）, （3）MIR＋Saline group（group C, n= 15）, （4）MIR＋lithium chloride group（group D, n= 15）. The B, C and D group were divided into 1 h, 3 h and 6 h subgroups with 5 rats in each subgroup. Flow cytometry was used to detecte the cadiocyte apoptosis rate. Apoptosis-related proteins Bcl-2, Caspase-3 were detected by the methods of RT-PCR and Western-blot. Result： Compared with group A, the cadiocyte apoptosis rate increased, Bcl-2 reduced, Casepase-3 increased significantly at 3 hours in group B and C. Whereas the cadiocyte apoptosis rate decreased in group D, and the expression of Bal-2 and Casepase-3 reached back to group A. Conclusion： Myocardial ischemia reperfusion injury induces increase of myocardial cell apoptosis, decrease of Bcl-2 and increase of Caspase- 3. Lithium chloride can relief above changes, suggesting that lithium chloride has protective effects against myocardial isehemia reperfusion injury.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2009年第6期468-471,共4页
Journal of Clinical Cardiology
