摘要
应用Ｓｏｕｔｈｅｒｎ杂交方法检测１５例原发性肝细胞癌及癌旁组织乙肝病毒（ＨＢＶ）存在状态，采用ＰＣＲ－ＳＳＣＰ（Ｐｏｌｙ－ｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ－ｓｉｎｇｌｅｓｔｒａｎｄｃｏｎｆｏｒｍａｔｉｏｎｐｏｌｙｍｏｒｐｈｉｓｍ）银染技术研究其ｐ５３基因突变。结果癌组织中１３例（８６．７％）有ＨＢＶＤＮＡ整合，同时３例伴游离ＨＢＶＤＮＡ；癌旁４例（２６．７％）有ＨＢＶＤＮＡ整合，而游离的ＨＢＶＤＮＡ却有６例。癌组织中ｐ５３基因变异８例（５３．３％），１例位于第５外显子，各有２例位于第６、第７外显子，３例位于第８外显子，癌旁组织未见ｐ５３基因异常，ｐ５３基因异常的癌组织中均有ＨＢＶＤＮＡ整合。研究结果表明，ＨＢＶＤＮＡ整合与ｐ５３基因突变密切相关，ＨＢＶＤＮＡ可能通过整合基因组后导致抑癌基因突变失去抑癌作用而引发肝癌。
In order to investigate the HBV DNA integration and p53 gene mutations in hepatocellular carcinoma (HCC), 15 specimens of HCC and their surrounding tissues (SL) were examined with southern blot hybridization and PCR SSCP P(polymerase chain reaction single strand conformation polymorphism). The results showed that integrated HBV DNA was found in 13 cases of HCC (86.7%) and 4 of SL (26.7%) respectively. Free HBV DNA was found in 3 cases of HCC and 6 of SL. The p53 gene abberation were detected in 8 specimens of HCC, one in exon 5, three in exon 8, two in exon 6 and exon 7 respectively. The tissues with p53 gene abberation were all accompanied by integrated HBV DNA. The results indicate that there is a correlation between p53 gene mutation and HBV DNA integration, suggesting that mutation of p53 gene would be one of hepatocarcinogenesis mechanisms in HBV infection.
出处
《实用癌症杂志》
1998年第2期95-97,共3页
The Practical Journal of Cancer
