摘要
以巨峰、玫瑰香、龙眼和泽香4个葡萄品种一年生扦插苗为试材,研究了氯化镉(CdCl2)处理后葡萄根系线粒体过氧化氢(H2O2)、膜通透性转换孔(MPTP)、膜电位(Δψ)、细胞色素C(Cytc)及根系活力的变化.结果表明:4个葡萄品种经0.5mmol·L-1的CdCl2处理后,根系线粒体H2O2含量上升,MPTP开放程度增大,Δψ降低,Cytc含量下降,根系活力明显降低;其中,H2O2含量和Cytc含量由高到低的顺序为:巨峰>泽香>玫瑰香>龙眼,而根系活力、MPTP和Δψ由高到低的顺序为:龙眼>玫瑰香>泽香>巨峰.表明巨峰葡萄根系活力比其他品种更易受到CdCl2的影响,而龙眼葡萄根系活力在4个品种中受CdCl2的影响最小.
Taking the one-year cutting-seedlings of four grape cuhivars ( Kyoho, Muscat Hamburg, Long Yan, and Ze Xiang) as test materials, their root mitochondrial hydrogen peroxide (H2O2 ) content, membrane permeability transition pore (MPTP), membrane potential (△ψ), cytochrome C ( Cyt c) content, and root activity were measured under effects of CdC12. For all test grape cuhivars, the root mitochondrial H2O2 content and MPTP increased, but the △ψ, Cyt c content, and root activity decreased after treated with 0. 5 mmol CdCl2 ·L-1. The mitochondrial H2O2 content and Cyt c content were in the sequence of Kyoho 〉 Ze Xiang 〉 Muscat Hamburg 〉 Long Yan, while the root activity, MPTP, and △ψ were in adverse, i. e. , Long Yan〉 Muscat Hamburg 〉 Ze Xiang 〉 Kyoho. Among the test grape cultivars, the root activity of Kyoho was easier to be inhibited under Cd stress, while that of Long Yan was least affected by CdCl2.
出处
《应用生态学报》
CAS
CSCD
北大核心
2009年第6期1390-1394,共5页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(30671452)资助
关键词
葡萄
根系活力
镉胁迫
线粒体特性
grape
root activity
cadmium stress
mitochondrial characteristics