摘要
ＤｅｔｅｒｍｉｎａｔｉｏｎｏｆａｍｉｔｒｉｐｔｙｌｉｎｅａｎｄｎｏｒｔｒｉｐｔｙｌｉｎｅｉｎｈｕｍａｎｌｉｖｅｒｍｉｃｒｏｓｏｍｅｓｗｉｔｈｒｅｖｅｒｓｅｄｐｈａｓｅＨＰＬＣｉｎｖｉｔｒｏ１ＳＨＵＹａｎ，ＺＨＵＲｏｎｇＨｕａ２，ＸＵＺｈｅｎＨｕ...
AIM: To develop a method for simultaneous determinations of amitriptyline (Ami) and its metabolite nortriptyline (Nor) in human liver microsomes. METHODS: An incubation buffer containing microsomes, NADPH generating system, and Ami, after termination of enzyme reaction and desipramine (Des) as internal standard (IS), was extracted with diethy ether and separated on a reversed phase ODS column. Detection was achieved at 242 nm by ultraviolet detector. RESULTS: No potential interfering peaks were found. Ami and Nor gave rapid elution and baseline resolution. The linear curves of both analyses ranged 0 02-10 nmol and the limit of detection was 0 01 nmol. The recovery (94 %-101 %) had good precision with relative s of <8 3 % . CONCLUSION: This method is rapid, sensitive, and simple for studying the metabolism of Ami and Nor.
出处
《中国药理学报》
CSCD
1998年第4期343-346,共4页
Acta Pharmacologica Sinica
关键词
阿米替林
去甲替林
高压液相色谱法
肝微粒体
amitriptyline
nortriptyline
high pressure liquid chromatography
liver microsomes
