摘要
【目的】分离和纯化Paenibacillus polymyxaCP7菌抗革兰氏阴性菌的活性组分,并鉴定其结构。【方法】采用固相萃取柱分离、阳离子交换层析、凝胶过滤层析和HPLC层析等技术对活性组分进行分离纯化,通过紫外-可见光谱、圆二色光谱、红外光谱和MALDI-TOF-TOF-MS质谱对所分离组分进行结构鉴定。【结果】从CP7菌发酵液中分离获得抗革兰氏阴性菌的B、C1和C2组分,分子量分别为1155、1169和1191D。并确定了C1组分的分子式为C53H100O13N16,结构由6个L-α,β-二氨基丁酸(DAB)、2个苏氨酸和2个亮氨酸组成,与多粘菌素E1的结构相同。【结论】从CP7菌发酵液中分离获得了3种抗革兰氏阴性菌的小分子多肽。经测试确认C1组分为多粘菌素类E1。推测B组分也是多粘菌素类的1个成员,而C2组分则可能是多粘菌素家族中的一个新成员。
[ Objective ] The components of anti-gram-negative bacteria by the strain CP7 of Paenibacillus polymyxa were purified, and the structure was identified. [Method] The antibacterial components from CP7 strain were purified by the solid phase extraction, cation-exchange chromatography, gel-filtration chromatography and HPLC. The components were analyzed with the technique of the ultraviolet-visible spectrum, circular dichroism spectroscopy, IR and MALDI-TOF-TOF-MS. [Result] Three components of anti-gram-negative bacteria, B, C1 and C2, were purified from Paenibacillus polymyxa CP7 broth. Their molecular weight was 1 155 D, 1 169 D and 1 191 D, respectively. The molecular formula of C1 was C53H100O13N16, which was composed by six DAB, two threonine and two leucine, with the same structure as polymyxin El. [Conclusion] Three kinds of small molecule peptide of anti-gram-negative bacteria were isolated from the CP7 fermentation broth. It is speculated that B was also a member of the polymyxin family, and C2 may be a new family member.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第6期2105-2110,共6页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目(30170717,30570036)
关键词
CP7菌
多粘类芽孢杆菌
抗菌物质
分离纯化
结构分析
strain CP7
Paenibacilluspolymyxa
polymyxin
isolation and purification
structure analysis
