摘要
选用90头新生"杜×长×大"三元杂交仔猪,随机分为3组,每组30头,分别为中药Ⅰ组(中药添加剂量10 g/kg),中药Ⅱ组(中药添加剂量20 g/kg),对照组(基础日粮组),试验期为31 d。45日龄时每窝随机选取6头仔猪屠宰后,分别采集胸腺和脾脏。采用反转录聚合酶链式反应(RT-PCR)方法,定量分析仔猪胸腺及脾脏中生长激素受体(GHR)mRNA的相对丰度。结果显示,与对照组相比较,2种剂量中药组的仔猪胸腺及脾脏中生长激素受体mRNA的含量均不同程度地提高,中药Ⅱ组效果更为显著。
Ninety newborn （Duroc×Landrace× Yorkshire） piglets were divides into 3 groups of 30 piglets each, Chinese native medicine group Ⅰ（dosage 10 g/kg）, Chinese native medicine group Ⅱ （dosage 20 mg/kg）, control group （basaldiet）,respectively. The trial was conducted for 31 d. At the end of the trial, 18 piglets （6 per group） were bled via the anterior vena cava. Thymus and spleen were collected to determine the comparative contents of GHR mRNA by RT-PCR. Results. showed that compared with the control group, the Chinese native medicine group Ⅰ and Ⅱ could remarkably enhance the comparative contents in GHR mRNA of piglets,and the effect of the Chinese native medicine groupⅡ was more remarkable.
出处
《动物医学进展》
CSCD
北大核心
2009年第6期66-69,共4页
Progress In Veterinary Medicine
