摘要
目的:初步建立人鼠嵌合乙肝动物模型。方法:将来源于人并嵌合有乙型肝炎病毒(HBV)全基因序列的肝癌细胞(HepG2.2.15,以下简称"2215细胞")经孕鼠子宫注射到孕期为15～18天的胎鼠腹中,诱导胎鼠对2215细胞产生免疫耐受,且在胎鼠出生后24h内经脾再次移植2215细胞。ELISA法检测不同时相点大鼠血清中的表面抗原(HBsAg)。结果:对照组与正常组大鼠的各时相点的HBsAg表达均为阴性,模型组大鼠第3、4、5、6、7、8、9、10、11、12、13、14、15、16周龄的HBsAg阳性率分别为60.5%、60.5%、58.1%、58.1%、48.8%、46.5%、47.6%、42.9%、35.7%、31.0%、31.7%、29.3%、19.5%、12.2%。结论:模型组中部分大鼠(48.27%)血清HBsAg的表达随着鼠龄的增加而减退,而另一部分大鼠(12.20%)可以将这种阳性表达持续到16周,表明该模型有长期表达的可能性。
Objective： To preliminarily establish the Hepatitis B animal model of tolerant rats with chimeric human hepatocellular cells which were integrated with HBV virus gene. Methods： The hepatoma cell （HepG2.2.15, shorter form：＂2215 cell＂ in the following） which was come from human and chimericed the was injected to the pregnant Wistar rats＇uteri, so the embryo of Wistar rats were got the immunotherapy to the 2215 cell, when the rats born, they were transplanted with the 2215 cell via spleen in 24hours. For all the groups, the surface antigen（ HB- sag） in the serum was tested with the method of ELISA in different time. Result ：The results showed that the positive ratio of HBsAg in model group with the age of 3,4,5,6,7,8,9,10,11,12,13,14,15,16 weeks was 60.5% ,60.5% ,58.1% ,58.1% ,48.8% ,46.5% ,47.6%, 42.9% ,35.7% ,31.0% ,31.7% ,29.3% ,19.5% and 12.2% respectively ,but the control and the normal group showed the negative ratio of HBsAg in different time. Conclusion： We found that the expression of HBsAg in the serum for part rats（48.27% ）of the model group decreased with the age increasing, but the other parts （ 12.20% ） could express the HBsAg till the age of 16 weeks, and we found that the model group was able to express the HBsAg for long time, so the experimental study provided reference for the further study which could keep the stationary of the model group.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2009年第2期126-128,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
广西科技厅项目(桂科能0630006-5A-04Z)
关键词
嵌合肝
肝癌细胞HepG2.2.15
大鼠
chimeric liver
human hepatocellular cells HepG 2.2.12
transplantation
