摘要
以拟南芥为供试材料,从形态指标、生理指标、分子指标3个层次研究了镉(Cd)的毒理效应,筛选Cd敏感生物标记物。分子指标的研究以18SrRNA为看家基因,错配修复基因MutS2homolog(atMSH2),atMSH3,atMSH7,细胞增殖核抗原1和2(atPCNA1和atPCNA2)为检测目的基因,采用半定量反转录-聚合酶链式反应(RT-PCR)技术研究Cd胁迫对上述错配修复相关基因表达的影响。结果表明,不同浓度(0、0.125、0.25、1.0、3.0mg·L-1)Cd处理7d后,根长随Cd胁迫强度的增加而降低;0.125mg·L-1Cd处理下,地上部可溶性蛋白含量显著增加,而在0.25、1.0和3.0mg·L-1Cd时降低,但仍高于对照;幼苗叶片数、地上部鲜重、叶绿素含量变化与对照相比差异均不显著。地上部atMSH2,atPCNA1,atPCNA2基因表达量的变化与Cd胁迫浓度呈明显的倒U字型关系,分别在0.125,0.25和0.125mg·L-1Cd时达到最大值。以上结果表明,地上部可溶性蛋白含量变化与上述3个错配修复相关基因表达量的改变趋势相符,且均对Cd污染胁迫较敏感,可以作为检测Cd污染及其相关生物学效应的潜在生物标记物。
Using A rabidopsis thaliana（Arabidopsis ） as tested material, the toxic effects of cadmium（Cd ） in cultural media on the three levels of morphological indexes, physiological indexes and molecular indexes were studied, in order to choose sensitive biomarkers indicative of Cd. The study on molecular indexes took 18S rRNA as house-keeping gene, MutS 2 homolog（atMSH2 ）, atMSH3, atMSH7, atPCNA 1 and atPCNA2 as targeted genes, which focused the effect of Cd on the above mismatch repair related genes expression of Arabidopsis seedlings with semi-quantitative Reverse Transcription-Polymerase Chain Reaction （RT-PCR）. The results indicated that the root length of Arabidopsis seedlings reduced with the increase of the concentration of Cd. The total soluble protein level of the shoots in Arabidopsis seedlings significantly increased by exposed to Cd of 0. 125 mg·L^-1 for 7 days, and reduced by exposed to Cd of 0.25, 1.0, 3.0 mg·L^-1 for 7 days whereas they were still higher than the level of control. Exposure to Cd of 0.125-3.0 mg·L^-1 did not have a significant effect on leaf numbers, fresh weight and chlorophyll level of the shoots in Arabidopsis seedlings as compared with the control. The inverted U-shaped dose-response effects of Cd on expression of atMSH2, atPCNA 1, atPCNA2 of the shoots of Arab idopsis seedlings were observed, with maximum effect at 0.125 mg·L^-1, 0.25 mg·L^-1 and 0.125 mg·L^-1 Cd, respectively. The above results indicate that the change trend of the total soluble protein level of the shoots in Arabidopsis seedlings was similar to that of above three mismatch repair related genes expression. They were all sensitive to the Cd pollution and could be served as potential biomarkers for the detection of Cd pollution and the related biological effects.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2009年第6期1246-1252,共7页
Journal of Agro-Environment Science
基金
中国科学院沈阳应用生态研究所陆地生态过程重点实验基金
中科院知识创新重大项目(KZCX1-YW-06-03)
国家重点基础研究发展计划(2004CB418506)
中俄自然资源与生态环境联合研究中心资助
