摘要
目的观察脊髓进行性压迫损伤(SCII)过程中神经细胞内钙离子(Ca2+)浓度、内质网跨膜激酶(Ire1)表达变化规律,探讨其在内质网应激过程中的意义。方法制作大鼠脊髓压迫模型,运用生物化学、免疫组化、Western blot、RT-PCR和图像分析等方法,观察SCII后脊髓神经细胞内Ca2+浓度、Ire1表达变化规律。结果SCII后神经细胞内Ca2+浓度于术后1、3、7、14、21、28d呈升高趋势,手术组与假手术组比较差异有统计学意义(P<0.05)。术后1d Ire1 mRNA和Ire1即有较高水平表达,术后3、7、14d迅速增高,术后21、28d逐渐下降,手术组与假手术组比较差异有统计学意义(P<0.05)。结论SCII导致细胞内Ca2+浓度升高而引起内质网应激。Ire1表达标志着内质网上未折叠蛋白积聚以及相关蛋白表达增加。表明Ca2+和Ire1都参与了内质网应激所致细胞凋亡信号转导的调节。
Objective To determine the expression changes of calcium concentration and Irel after spinal cord ischemia/reperfusion injury（SCⅡ） in the rat,so as to investigate their role in endoplasic reticulum stress. Methods By using experimental SCⅡ in the rat. The calcium concentration and Irel express level was detected by biochemistry method, immunohistochemistry, western blotting,RT-PCR and imagy-analysis after SCⅡ in the rat. Results After progressive compression of spinal cord,the Ca^2＋ concentration in neurons was increased respectively in 3,7,14 d after operation. There was a significant difference between operation group and control group （P〈0.05）. The expression of Irel mRNA and Irel was obviously increased in 1 d after operation, and rapidly elevated in 3,7,14 d after operation, and then the expression of Irel mRNA and Irel was decreased in 21,28d after operation. There was a significant difference between the operation group and the control group （P〈0.05）. Conclusion The increase of Ca^2＋ concentration in the neurons might he a factor trigering the endoplamic reticulum stress induced by progressive compression spinal cord. The increase of Irel expression suggest that the unfold protein accumulated in the ER,indicating the neurons were in the stage of ERS. In summary,the above mentioned results imply that Ca^2＋ ,Irel might participate in the signal transduction of apoptosis induced by endoplasmic reticulum stress in progressive compression spinal cord ischemic injury.
出处
《重庆医学》
CAS
CSCD
北大核心
2009年第12期1459-1461,I0001,共4页
Chongqing medicine
基金
国家自然科学基金资助项目(302702437)
2003年教育部博士点基金资助项目(2004-7)
关键词
钙离子
内质网跨膜激酶
脊髓进行性压迫性损伤
calcium ion, Inositol reguiring enzyme, progressive compression spinal cord ischemic injury
