摘要
Ficoll法分离大鼠外周血淋巴细胞,于42℃、5%CO2培养箱中热休克处理90min,采用流式细胞仪检测热休克预处理后恢复不同时间的淋巴细胞中HSP70的表达情况;采用流式细胞术AnnexinV/PI双标法检测经过热休克预处理的淋巴细胞经0.5Gy、1.0Gy、1.5Gy、2.0Gy、2.5Gy、3.0GyX射线辐照后细胞凋亡率的变化。以探讨热休克预处理后淋巴细胞中HSP70的表达情况和HSP70对X射线辐照后淋巴细胞凋亡率的影响。结果显示:1.淋巴细胞经热休克预处理后恢复1h,HSP70表达即有明显增加,4h达到高峰,可持续表达24h。2.热休克预处理诱导表达的HSP70可以抵抗X射线(1.0～3.0Gy)诱导的淋巴细胞凋亡。由此本工作可以得出结论,热休克预处理表达的HSP70可抵抗X射线诱发的淋巴细胞凋亡。
In this paper the Ficoll method was used to isolate peripheral blood lymphocyte of rats, and then the isolated lymphocytes were treated by heat shock for 90 min under 42℃ and 5 % CO2. Flow cytometer was applied to detect the expression of HSP70 after heat shock treatment at different revival time. Flow cytometry Annexin V/PI was u ＂tllized to measure the apoptosis rate of lymphocytes which were already pretreated by heat shock and irradiated under 0.5 Gy, 1.0 Gy, 1.5 Gy, 2.0 Gy ,2.5 Gy, 3.0 Gy X-ray. The expression of HSPT0 after heat shock treatment and the influence of HSP70 to the apoptosis rate of lymphocytes irradiated under different doses has been investigated. The results show that the expression of HSP70 in lymphocytes increases obviously at lh and reaches the maximum at 4 h after heat shock treatment, and can maintains in 24 h. The expression of HSP70 induced by heat shock pretreatment can counteract lymphocyte apoptosis produced in irradiation by X-ray （1.0-3.0 Gy）. So the irradiation by X-ray is a useful method to confirm counteraction of lymphocyte apoptosis by HSP70.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
2009年第3期156-160,共5页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金(10275083)资助
