摘要
采用胸腔注射植物血球凝集素及秋水仙素溶液,取活体头肾细胞经低渗、固定、空气干燥法,分析了条石鲷、金钱鱼和星斑裸颊鲷的核型,并进行了比较。试验结果表明,3种海水鱼中期染色体均为二倍体,且未发现异型性染色体、随体和次缢痕。其核型如下:条石鲷2n=48,2m+2st+44t,臂数:NF=52;金钱鱼2n=48,48t,臂数:NF=48;星斑裸颊鲷2n=48,48t,臂数:NF=48;条石鲷的核型与后两者存在微小差异。
The karyotypes of 3 marine important fish species were obtained from metaphase kidney cells by intraperitonal injection of PHA and colchicine using hypatoic-air technique, under lower osmotic pressure. The results showed that the 3 species were diploid chromosome style, and no heteromorphic sex chromosomes, along with bodies or hypo-split mark. The karyotype formula are found as follows： the karyotype in striped knifejaw Oplegnathus fasciatus is 2n=48, 2m＋2st＋44t, NF=52. The karyotype in spotted scat Scatophagus arguss is 2n=48, 48t, NF= 48. The karyotype in spangled emperor Lethrinus nebulosus is 2n=48,48t,NF=48.
出处
《水产科学》
CAS
北大核心
2009年第6期325-328,共4页
Fisheries Science
基金
广东省科技计划项目(200651900028)
广州市属高校科技计划项目(2037)
广州市教育局高校校外实习基地项目(90-2040)
广州大学产学研基金资助项目(1001-1030)
广州大学创新团队项目
关键词
条石鲷
金钱鱼
星斑裸颊鲷
染色体
核型
Oplegnathus fasciatus
Scatophagus argus
Lethrinus nebulosus
chromosome
karyotype
