摘要
目的:研究在离体条件下,Klebsilla ASP-15对某些植物病原真菌的拮抗作用,为具有PGPR功能菌株的进一步应用提供理论依据。方法:平板对峙培养进行比对实验,初步研究ASP-15的抑病能力与抑病机理。结果:ASP-15菌对除尖孢镰刀菌外其他6株被测试菌的抑制率达57.8%～84.8%以上,对尖孢镰刀菌的抑制率为17.7%;含菌体的发酵液抑菌效果最好;在培养5～7时,稀释200倍的发酵液的抑菌率从45%降到了15%,稀释200倍的发酵上清液的抑菌率从8%降到了1%;ASP-15的生长引起了病原菌丝生长发生改变,使菌丝变粗,菌丝的分裂点减少,在分隔处有膨大突起现象,菌丝里的原生质体减少,孢子无大量生长。结论:ASP-15菌具有较广谱的抑制植物真菌病害的能力,但其生防作用主要还是营养竞争,抢先占领生态位引起的。
Objective：In the vitro conditions, some plant pathogenic fungi were resisted by Klebsilla ASP-15 with the PGPR function strains in order to provide a theoretical basis for further applications. Method： Confrontation growth between the culture plate was used in order that the ASP - 15 ability to suppress disease and mechanism of disease suppression were made preliminary research. Result：Tile ASP- 15 could resist the growth of other six strains except Fusarium oxysporum on inhibition rate of 57.8% - 84.8% more than of Fusarium oxysporum on the inhibitory rate of 17.7 %. Bacterial fermentation broth containing the bacteriostafic effect was the best. In the development of 5 days to 7 days, when the inhibition rate of 200 times dilution of fermentation broth was reduced from 45% to 15%, the inhibitory rate of 200 times dilution of the fermentation supematant was reduced from 8% to 1%. ASP- 15 led to the growth of pathogenic changes in the growth of silk, so that mycelium was thicken, the split - point of myeelitan were reduced, in the separation of processes there was some expansion, the mycelial protoplast was reduced without a large number of spore growth. Conclusion： ASP - 15 strain has a more broad - spectrum inhibition of the ability of plant fungal diseases, but its role in biocontrol is mainly nutritional competition, the first arising from the occupied niche.
出处
《生物技术》
CAS
CSCD
北大核心
2009年第3期55-58,共4页
Biotechnology
基金
黑龙江省科学院青年创新基金项目资助
