摘要
目的：研究双苄基异喹啉化合物Ｙｓ９６对阿霉素或长春新碱耐药株人癌细胞抗药性的逆转作用。方法：在体外培养的药物敏感株ＭＣＦ７及其阿霉素耐药株ＭＣＦ７／Ａｄ以及药物敏感株ＫＢ及其长春新碱耐药株ＫＢＶ２００的体外试验系统中，采用ＭＴＴ法研究耐药株人癌细胞在Ｙｓ９６作用下对阿霉素或长春新碱敏感性（ＩＣ５０）的变化。结果：耐药株ＭＣＦ７／Ａｄ和ＫＢＶ２００在Ｙｓ９６（１００μｍｏｌ／Ｌ）的作用下，阿霉素和长春新碱对其ＩＣ５０值分别接近于二药单独用药时对敏感株ＭＣＦ７和ＫＢ的ＩＣ５０。结论：Ｙｓ９６基本上能完全逆转ＭＣＦ７／Ａｄ或ＫＢＶ２００对阿霉素或长春新碱的抗药性。
Objective: Reversal effect of Ys96,a bisbenzylisoquinoline,on the resistance of human cancer cells to adriamycin or vincristine was studied in vitroMethod:In an in vitro culture system of human cancer cells MCF7 and its adriamycinresistant line MCF7/Ad or KB and its vincristineresistant line KBV200,the sensitivity(IC50)of the resistant cell lines to adriamycin or vincristine was evaluated with a MTT assay.Result:IC50value of adriamycin or vincristine in combination with Ys96 at a concentration of 1.00μmol/L against MCF7/Ad or KBv200 was found to be close to that of adriamycin alone or vincristine alone against the sensitive cell line MCF7 or KBConclusion:The drug resistance of MCF7/Ad or KBv200 could be essentially reversed by Ys96 at a concentration of 100μmol/L
出处
《中国中药杂志》
CAS
CSCD
北大核心
1998年第7期427-428,共2页
China Journal of Chinese Materia Medica
基金
国家自然科学基金
关键词
双苄基异喹啉
阿霉素
抗癌
多药抗药性
汉防己
bisbenzylisoquinoline
adriamycin
vincristine
anticancer activity
multidrug resistance
