摘要
目的拟从观察电磁脉冲（ＥＭＰ）辐照后大鼠小脑、海马中一氧化氮合酶（ＮＯＳ）的表达情况，探讨ＥＭＰ所致大鼠学习、记忆能力改变的可能机制。方法采用ＳＰ免疫组织化学染色检测ＥＭＰ辐照后大鼠小脑、海马组织中ＮＯＳ阳性神经元的变化。结果ＥＭＰ辐照后１．５、２４ｈ海马中ＮＯＳ阳性神经元数目较对照组显著下降，且着色变浅，照后４８ｈＮＯＳ阳性神经元数目恢复至对照水平，但着色仍浅。小脑中ＮＯＳ阳性细胞在照后无明显变化。结论ＮＯＳ在ＥＭＰ所致大鼠学习。
Objective In order to explore the role of nitric oxide in the obstruction of learning and memory of the rat caused by exposing to electromagnetic pulse(EMP),the distribution of nitric oxide synthase(NOS) expression was studied in hippocampus and cerebellum of the rat following exposure to EMP. Methods SP immunohistochemical staining was employed to detect the distribution of NOS expression. Results The number of NOS positive neurons and the intensity of positive staining in hippocampus were decreased at 1.5 and 24h after exposure to EMP. At 48h,the number of NOS positive neurons reversed to control level but the intensity of positive staining was still lower.The expression of NOS in cerebellum had no obvious changes.Conclusion Decrease of NOS expression in hippocampus relates to the obstruction of learning and memory of the rat after exposure to EMP.
出处
《中华物理医学杂志》
CAS
CSCD
1998年第2期81-83,共3页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
电磁学
海马小脑
一氧化氮
代谢
electromagnetics
hippocampus/rad eff
cerebellum/rad eff
nitric oxide/metab
immunoenzyme technics
