摘要
观察谷氨酸单钠（ＭＳＧ）对大鼠肾上腺酶和脂类的影响。动物分为新生期（１１ｄ）、青春期（５２ｄ）、成年期（１００ｄ）３组。用酶组织化学方法显示琥珀酸脱氢酶（ＳＤＨ）、乳酸脱氢酶（ＬＤＨ）、３β羟甾脱氢酶（３βＨＳＤＨ）、葡萄糖６磷酸酶（Ｇ６Ｐａｓｅ）、葡萄糖６磷酸脱氢酶（Ｇ６ＰＤＨ）、非特异性酯酶（ＮｓＥ）、三磷酸腺苷酶（ＡＴＰａｓｅ），用油红Ｏ法显示脂类。用半定量和显微分光光度计进行定量测定。结果：１１ｄ，ＭＳＧ组上述指标高于对照组；而５２ｄ和１００ｄ，ＭＳＧ组的ＬＤＨ高于对照组。
sing MSG lesioned rats, the changes of enzymes and lipid in rat′s adrenal gland were studied . Animals were seperated into three groups of 11th day (newborn stage), 52th day (pu terty ) and 100th day (adult). Each stage rats were seperated into two subgroups, the sham and lesion subgroups. The speciments of adrenal glands were stained with histochemistry and red oil O to show SDH,LDH,3 β HSDH,G 6 Pase,G 6 PDH,NsE and lipid. Quantitative analyses were performed. The present study revealed that all of the above enzyme activities and lipid were elevated in lesion group of 11th day. In lesion group of 52th day and 100th day, the enzyme activities of LDH were elevated and other enzyme activities and lipid were low. These studies reveal that MSG may poisined on adrenal gland.
出处
《首都医科大学学报》
CAS
1998年第2期109-112,共4页
Journal of Capital Medical University
关键词
谷氨酸单钠
组织化学
肾上腺
monosodium glutamate
histochemistry
adren gland
rat
