摘要
目的:建立芹菜素的高效液相色谱检测方法,研究芹菜素与蛋白的相互作用。方法:采用C8柱(250mm×4.6mm,5μm)进行色谱分析,梯度洗脱,检测波长337nm,流速1.0mL·min-1;同时,结合平衡透析法,研究了芹菜素与蛋白的相互作用。结果:在2min之内实现了芹菜素的快速检测,并且在37℃的水浴恒温培养4h后,芹菜素与蛋白发生相互作用,最大结合量为5.55,结合常数为0.17(mg·L-1)-1。结论:本法可以快速准确地检测芹菜素,并可研究其与蛋白的相互作用。
Objective： To establish an HPLC method for the determination of apigenin and investigate its interaction with human serum albumin （HSA）. Methods：Apigenin was detected by high performance liquid chromatography with Cs column （250 mm×4. 6 mm,5 μm） and gradient elution under the detection wavelength of 337 nm and the rate of 1.0 mL·min^-1. Furthermore,its interaction with human serum albumin was investigated by equilibrium dialysis. Results：Apigenin was immediately detected in 2 min and a strong interaction between these two substances was found after 4 -hour culture in 37 ℃ water bath,with a binding constant of 0. 17 （mg · L^-1） -1 and the maximum binding amount to 5.55. Conclusion：The method is accurate, sensitive for the determination of apigenin, and can be used to investigate the interaction of human serum albumin with other pharmaceuticals.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第6期975-978,共4页
Chinese Journal of Pharmaceutical Analysis
基金
华南农业大学校长基金
化学生物传感与化学计量学国家重点实验室开放基金资助
