摘要
目的:建立测定罗布麻叶中金丝桃苷和罗布麻甲素含量的方法。方法:采用Zorbax-C18(4.6mm×150mm,5μm)色谱柱,以乙腈-1.0%醋酸溶液(14∶86)为流动相,流速0.8mL·min-1,检测波长360nm,柱温30℃。结果:金丝桃苷和罗布麻甲素的线性范围分别为0.024～0.487μg和0.028～0.551μg;平均回收率(n=9)分别为98.7%和99.8%。结论:所建立的HPLC法可用于同时测定罗布麻叶中金丝桃苷和罗布麻甲素的含量。
Objective：To establish the quantitative analysis of hyperoside and isoquercitrin by RP - HPLC. Methods：The determination of hyperoside and isoquercitrin was conducted by RP - HPLC using a Zorbax - C18（4. 6 mm × 150 mm,5 μm）column. The mobile phase was acetonitrile -1.0% acetic acid solution（ 14： 86） with the flow rate of 0. 8 mL · min^-1, the detection wavelength was 360 nm, and the column temperature was set at 30 ℃. Results ： In HPLC analysis, the linear ranges of hyperoside and isoquercitrin were 0. 024 - 0. 487 μg and O. 028 - 0. 551 μg, respectively ; The average recoveries （ n = 9） were 98.7 % and 99.8 %, respectively. Conduslon ： The established method can be used to determine the contents of hyperoside and isoquercitrin in Apocynum venetum L. simultaneously.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第6期1001-1003,共3页
Chinese Journal of Pharmaceutical Analysis
