摘要
目的：建立虾仁中甲醛的高效液相色谱测定法。方法：准确称取捣碎的试样约5 g（精确至0.01 g）,用水提取样品中的甲醛,经过滤后,与2,4-二硝基苯肼衍生,在波长365 nm处以紫外检测器检测,外标法定量。结果：加标回收率在94.4%～99.9%,相对标准偏差均小于2%,检出限小于0.20 mg/kg。结论：本法分离度好,灵敏度高,操作简便,适用于虾仁中甲醛的测定。
Objective： To develop a method for determination of formaldehyde in shrimp by HPLC chromatography with UV detector. Methods：The formaldehyde in 5 gram pounding sample was extracted by water and derivatized with 2, 4 dinitrophenylhydrazine, then detected by UV detector with the wavelegth at 365 nm. Then it was analyzed by external calibration method. Results：The recoveries were between 94. 4% -99. 9% , the lowest detection limit 0. 20 mg/kg and relative standard deviation below 2%. Conclusion： The method is simple and convenient with good separation for analysis of formaldehyde in shrimp.
出处
《中国卫生检验杂志》
CAS
2009年第6期1289-1290,共2页
Chinese Journal of Health Laboratory Technology
