摘要
以工业酿造常用菌株米曲霉沪酿3042为出发菌株,采用紫外诱变和氯酸钾压迫相结合的方法,得到硝酸盐营养缺陷型(niaD-)宿主菌,将含有niaD基因的载体转化到该宿主中,转化率为0.7个转化子/μgDNA,相对比较低,但转化子传代数次后遗传仍很稳定,该转化体系可用于工业生产菌沪酿3042的遗传改造。
Aspergillus oryzae HuNiang 3042 was used as starting strain, and the host mutant strain with nitrate auxotrophic was screended by UV-induction and kalium chloricum resistance.The vector with the niaD gene coding for nitrate reductase was transfered to host strain.The transformants can use sodium nitrate as sole nitrogen source.The frequency of transformation was relatively low （0.7 transformants/μg DNA）, but the transformed phenotype of transformants was extremely stable for many generations without selective pressure. So this transformation system can be applied for genetics modification of Aspergillus oryzae HuNiang 3042.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第6期94-95,99,共3页
Science and Technology of Food Industry
基金
广东省科技计划项目(2007A010900001)
关键词
米曲霉
niaD^-
筛选
转化
Aspergillus oryzae
nitrate auxotrophic mutant
screening
transformation
