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鸡蛋清中卵转铁蛋白的分离纯化 被引量：3

Separation and purification of ovotransferrin in hen egg white
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摘要 鸡蛋清经去卵粘蛋白、盐析后,获得卵转铁蛋白粗提物,通过两步Q-Sepharose Fast Flow离子交换色谱纯化卵转铁蛋白(OVT),利用SDS-PAGE电泳和HPLC法检测其纯度。结果表明,两步阴离子交换色谱获得纯度分别为78.26%和97.53%的卵转铁蛋白。 The ovotransferrin was purified by mucin-free and ammonium sulfate precipitation and two-step Q- Sepharose Fast Flow ion-exchange chromatograph from the hen egg white, the purity of ovotransferrin was assayed by SDS-PAGE and HPLC.The results showed the purity of ovotransferrin after two-step Q-Sepharose Fast Flow anion-exchange chromatograph was 78.26% and 97.53% respectively.
作者 徐明生 杜华英 蒋艳 吴少福 曾婷婷 陈琴
机构地区 江西农业大学食品科学与工程学院
出处 《食品工业科技》 CAS CSCD 北大核心 2009年第6期209-211,共3页 Science and Technology of Food Industry
基金 江西省教育厅项目(gjj98134)
关键词 卵转铁蛋白 阴离子交换层析 纯化 ovotransferrin anion-exchange chromatograph purification
分类号 TS252.1 [轻工技术与工程—农产品加工及贮藏工程]
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参考文献11

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同被引文献39

  • 1赵新淮,冯志彪.大豆蛋白水解物水解度测定的研究[J].东北农业大学学报,1995,26(2):178-181. 被引量：177
  • 2曹健,李卫林,张磊,汤克勇.酶解胶原蛋白产物分子量的控制研究[J].食品工业科技,2006,27(8):85-88. 被引量：10
  • 3王庆华,钟细娥,吕振岳.卵清转铁蛋白的提取[J].食品工业科技,2006,27(12):110-111. 被引量：1
  • 4陈杰,马美湖.风味蛋白酶水解蛋清工艺条件的研究[J].现代食品科技,2007,23(7):43-44. 被引量：19
  • 5Thomas B Osborne, George F CamPbell. The Protein constituents of egg white [J]. Journal of Ameriean Chemieal, 1900, 22(7): 422-450.
  • 6Francesco Giansanti, Loris Leboffe, Giuseppina Pitari, et al. Physiological roles of ovotransferrin [J]. Biochimica et Biophysica Acta, 2012, 1820: 218-225.
  • 7WU J P, Alexandra Acero-Lopez. Ovotransferrin: Structure, bioactivities, and preparation [J]. Food Research International, 2012(46): 480-487.
  • 8Dubiard C Guerin, Pasco M, Hietanen A, et al. Hen egg white fractionafion by ion-exchange chromatography [J]. Journal of Chromatography A, 2005, 1090: 58-67.
  • 9Yuan W, Zhao G F, Dong X Y, et al. High-capacity purification of hen egg-white proteins by ion-exchange electrochromatography with an oscillatory transverse electric field [J]. Sep sci, 2006(29): 2383-2389.
  • 10Vachier M C, Piot M, Awadr A C. Isolation of hen egg white lysozyme, ovotransferrin and ovalbumin, using a quaternary ammonium bound to a highly crosslinked agarose matrix [J]. Journal of Chromatography B, 1995(664): 201- 210.

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食品工业科技
2009年 第6期

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