摘要
目的:利用响应面分析法对楮实多糖的提取工艺进行优化。方法:在单因素实验基础上选取实验因素与水平,根据中心组合(Box-Benhnken)实验设计原理采用四因素五水平的响应面分析法,依据回归分析确定各工艺条件的影响因子,以楮实多糖提取率为响应值作响应面和等高线,并分析各个因素的显著性和交互作用。结果:楮实多糖水浸提的最佳工艺条件为:料液比为1:5.3,提取时间为3h,提取次数为4次,提取温度为96.5℃。结论:在最佳工艺条件下,楮实多糖的实际提取率为6.03%。
Objective：The technology for extracting crude Broussonetia papyrifera polysaccharide was optimized by response surface methodology（ RSM）.Methods：On the basis of single factor experiments,the optimum conditions for the extraction of polysaccharide were obtained through Box-Benhnken center-united experiment design and response surface methodology with 4 factors and 5 levels.Then the factors influencing the technological parameters were determined by regression analysis. Response surface and contour were finally graphed with the extraction rate as the response value.Results：The optimum conditions of extracting Broussonetia papyrifera polysaccharide were as follows：the ratio of material to solvent of 1：5.3 （w/v）, extraction time of 3 h ,4 times extraction and extraction temperature of 96.5℃.Conclusion ：The yield of polysaccharide is 6.03% under the optimum conditions.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第6期218-221,共4页
Science and Technology of Food Industry
基金
国家自然科学基金项目(30760297)
973计划前期研究专项(2007CB516813)
贵州省中药现代化科技产业研究开发专项(黔科合农字(2006)5041号)
关键词
楮实
多糖
提取
响应面分析
Broussonetia papyrifera
polysaccharides
extraction
response surface methodology （ RSM ）
