摘要
基于表面等离子体共振(Surface plasmon resonance,SPR)原理的基因芯片检测方法不需要标记,是一种很有潜力的高通微量分析(High-throughput microanalysis,HTMA)方法。应用自组装单分子层技术(Self-as-sembled monolayer,SAM)制备淋病奈瑟菌探针点阵的基因芯片,应用自行组建的SPR和表面等离子体共振成像(SPR imaging,SPRI)基因芯片分析系统,对该基因芯片进行检测分析,研究基因芯片上探针点阵的杂交反应。结果表明:SPR共振曲线上都有明显的共振吸收峰,探针杂交后折射率增大,共振角增大,分子量增大。由SPR检测界面或共振曲线可判断待分析样本与基因芯片上的探针是否发生杂交反应,待分析样本中是否含有待检测的物质。由SPRI检测系统可实时、直观地观察基因芯片上的探针是否发生杂交反应。SPR和SPRI系统可进行定性和定量分析。
The detection method of gene chip based on SPR principle is a potential high-throughput microanalysis method without labelling. With the use of Self-assembled monolayer （SAM） technology, the gene chip of Neisseria gonorrhoeae probe lattice has been prepared, detected and analyzed using the Surface plasmon resonance （SPR） and SPR imaging （ SPRI） gene chip detection system here-in provided for research in the hyhridizatin reaction on the probe lattice of gene chip. The result indicates that there is an obvious resonance assimilate peak on the SPR resonance curve. And after hybridization, the refractive index and resonance as well as molecular weight of the probe have increased. So whether a hybridization takes place or whether the wanted ingredient is in the sample under examination can be determined by using SPR to watch the detecting interface or the resonance curve. The SPRI detection system is available for observing the happening of a hybridization on the probe of gene chip in real-time and straighforwardly. The SPR and SPRI system can do analysis qualitatively and quantitatively.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2009年第3期653-656,共4页
Journal of Biomedical Engineering
基金
广东省自然科学基金资助项目(845106320100007605006063)
关键词
奈瑟菌
淋病
表面等离子体共振表面等离子体共振成像
基因芯片
Neisseria gonorrhoeae Surface plasmon resonance （SPR） Surface plasmon resonance imaging （SPRI） Gene chip
