摘要
目的利用N．乙基-N’-[（3-二甲氨基）丙基]碳二亚胺盐酸盐（EDC）作为催化剂，乙二胺作为交联剂制备新型海藻酸钠组织工程支架材料（简称材料）并测试其细胞相容性。方法以体外培养的人成纤维细胞作为对象，采用四甲基偶氮唑盐（MTT）法检测材料浸渍液对细胞增殖情况的影响，光镜下观察材料浸渍液中细胞的生长状况。将人成纤维细胞悬液接种于材料表面，制备人成纤维细胞一材料复合物，扫描电镜下观察培养7d的复合物表面细胞的黏附和生长状态，评价材料的细胞相容性。结果材料浸渍液作用1、2、4、7d后人成纤维细胞的相对增殖率（RGR）分别为98．00％、104．10％、110．80％、93．17％，毒性均为0级或1级。倒置显微镜观察人成纤维细胞在材料浸渍液中生长形态良好。扫描电镜下培养7d的人成纤维细胞．材料复合物表面人成纤维细胞能很好的与材料黏附，材料表面细胞均生长旺盛形成许多伪足状突起，并分泌产生细胞基质。结论新型海藻酸钠组织工程支架材料与人成纤维细胞的相容性良好，为其作为细胞生长支持物和进一步的医学应用提供了实验依据。
Objective To prepare neotype sodium alginate tissue engineering scaffold stuff（stuff for short） with ethylene diamine as cross linking agent and EDC as catalyzer, and to examine the cell compatibility. Methods Human fibroblasts were cultured in vitro, and the effect of the stuff impregnating solution on cell proliferation was evaluated by methyl thiazolyl tetrazolium （MTT） method. Cell growth situation in stuff impregnating solution was observed by microscopy. Human fibroblasts suspension were seeded on the surface of stuff to prepare human fibroblasts and the stuff complex. After 7 days, the cells adhesion to the complex surface and their growth status were observed by scanning electron microscopy, and cell compatibility of the stuff was evaluated. Results After 1,2,4,7 days in stuff impregnating solution, the relative growth rates （RGR） were 98.00%, 104.10%, 110.80%, 93.17% respeetively and toxicities to human fibroblasts of the stuff were all 0 or 1 grade. Human fibroblasts grew well in stuff impregnating solution under invert microscope. Human fibroblasts had good adhesion to the stuff on the complex surface after 7 days in scanning electron microscopy, and the cells on the stuff surface grew well to form many pseudopodia and secreted cell matrix. Conclusion The neotype alginate tissue engineering scaffold stuff has good cell compatibility with the human fibroblasts, which can provide cell growth supplement and experimental evidence for further medical application.
出处
《中华生物医学工程杂志》
CAS
2009年第1期27-31,共5页
Chinese Journal of Biomedical Engineering
关键词
海藻酸钠
组织工程
支架
成纤维细胞
细胞相容性
Sodium alginate
Tissue engineering
Stents
Fibroblasts
Cell compatibility
