摘要
目的探讨糖皮质激素对心肌缺血-再灌注损伤的保护作用。方法将18只家兔应用冠状动脉左前降支结扎术造成心肌缺血模型,随机分成3组:心肌缺血再灌注损伤模型组(模型组)、糖皮质激素一次大剂量保护组(大剂量组)以及糖皮质激素多次小剂量保护组(小剂量组),每组6只。应用BL-420生物信号采集系统观察记录心电图ST段改变,分别在缺血前、缺血15 min以及再灌注30 min取血测定血浆丙二醛(MDA)浓度;缺血区心肌标本制作冰冻切片,行组织化学染色,观察缺血区琥珀酸脱氢酶活性变化。结果糖皮质激素的大、小剂量保护组再灌注过程中ST段恢复的时间较短,快于模型组;大剂量组血清MDA水平低于小剂量组(P<0.05),且两组均低于模型组(P<0.01);大剂量组缺血心肌琥珀酸脱氢酶活性高于小剂量组,且两组均明显高于模型组。结论糖皮质激素对心肌缺血-再灌注损伤可以起到有效的保护作用,一次大剂量的治疗保护效果要好于多次小剂量。
Objective To study the protective effect of glucocorticoid preconditioning on the myocardial ischemic and reperfused hearts. Methods Totally 18 rabbits were randomly divided into three groups： myocardial ischemia-reperfusion model （model）, high-dose glucocorticoid given by one time group （high-dose group） and low- dose glucocorticoid given by several times group （low-dose group）, with six rabbits in each group. Myocardial ischemia was induced by left anterior descending coronary artery ligation. ST segments were recorded by the BL-420 biological signal acquisition system. Plasma malondial dehyde （MDA） was examined before ischcmia, at 15 min after ischemia and 30 min after reperfusion; ischemic heart muscles were prepared with cryotomy and stained histochemically. Succinic dehydrogenase activity was observed in the ischemic region. Results There was shorter time of ST-segment recovery in the high-dose group and the low-dose group than that in the model group. Serum level of MDA in the high-dose group was lower than in the low-dose group （P〈0.05）, and both were also lower than that in the model group （P〈0. 01）. The succinate dehydrogcnasc activity in the high-dose group was significantly stronger than in the low-dose group, and both were significantly stronger than that in the model. Conclusion Glucocorticoid plays an effectively protective role in myocardial ischemia-reperfusion injury. The protective effect of high dose is better than low-dose therapy.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2009年第3期292-294,共3页
Journal of Xi’an Jiaotong University（Medical Sciences）
关键词
糖皮质激素
心肌
缺血-再灌注损伤
家兔
glucocorticoid
cardiac muscle
ischemica reperfusion injury
rabbit
