摘要
为了探讨聚合酶链反应在牛血清支原体检测上的应用价值,以支原体高度保守的rRNA操纵子(支原体基因组中16SrRNA的编码区序列)设计引物,采用碱裂解法提取牛血清中支原体DNA作为模板进行聚合酶链反应。结果表明,阳性、阴性和内控对照都扩增出了预期的条带,聚合酶链反应与支原体培养法比较,有灵敏、快速、特异性高的特点,可用于牛血清中支原体的常规检测。
To study the application value of the polymerase chain reaction to detect mycoplasma in bovine serum. The primer was designed accordance with the highly conserved rRNA operon of the mycoplasma （ the 16SrRNA coding region in the mycoplasma genome）, the mycoplasma DNA was extracted from bovine serum using alkaline lysis method, as the complete, DNA was amplified by PCR. The results showed that positive, negative and internal control had been amplificated the expected bands. PCR is more sensitive, rapid and highly specific method for detecting the mycoplasma in bovine serum compared to the mycoplasma culture method. The PCR is a valuable tool for conventional detecting the mycoplasma in bovine serum.
出处
《微生物学免疫学进展》
2009年第2期36-39,共4页
Progress In Microbiology and Immunology
关键词
聚合酶链反应
支原体
内控对照
Polymerase chin reaction （PCR）
Mycoplasma
Internal control
