摘要
目的:研究苦参碱对人肝癌细胞系体外诱导分化作用,并探讨其作用机制。方法:不同浓度苦参碱体外作用于Bel-7404细胞,噻唑蓝比色法(MTT法)绘制生长曲线,光镜下观察细胞形态变化,分别以软琼脂集落形成试验、细胞黏附试验、运动试验、侵袭试验检测细胞生物学特性变化,以流式细胞仪分析细胞周期变化。结果:0.5-1.0g/L苦参碱作用于Bel-7404细胞后,明显抑制细胞增殖,细胞形态向正常转化,克隆形成率显著降低,细胞黏附力明显上升,运动力和侵袭力则显著下降。细胞周期分析G1期细胞堆积,G2和S期细胞显著降低。结论:一定浓度的苦参碱具有抑制Bel-7404细胞生长增殖和诱导分化作用,其作用机制可能与细胞周期调节有关。
Objectives： To investigate the differentiation of human hepatocellular carcinoma cell line Bel-7404 induced by Matrine in vitro, and discuss its mechanism. Methods： After dealing human hepatocellular carcinoma cell line Bel-7404 with different concentrations of Matrine, we drew cell growth curve by MTT colorimetry, and cell morphology was examined by light microscope. Then we evaluated the tumor cell biological characteristics changes through improved soft agarose clone forming test, cell adhesion assay, cell movement assay and cell invasion assay. Also we detemined cell cycle distributing by flow cytometry. Results： After 72 hours being treated with 0.5, 0.75, 1.0g/L, in the Matrine test groups, the cell proliferation was inhibited significantly, and cell shape transformed to normal. The amounts of clone were remarkably reduced. The cell adhesion ability increased in different level. At the same time cell ability of movement and invasion differently weakened. Analysis of cell cycle shown that along with rising of Matrine concentration, the cell amounts in G~ phase increased accordingly and cell amounts in G： and S phase remarkbly decreased. Conclusion： During the appropriate concentration range, Matrine not only inhibited Bel-7404 growth and proliferation, but also induced tumor cell to differentiate. Its probably mechanism may related to cell cycle regulation.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2009年第7期955-958,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
浙江省医药卫生科研基金项目(No.2003B07)
关键词
苦参碱
肝细胞癌
诱导分化
Matrine
Hepatocellular carcinoma
Induced differentiation
