摘要
为了实现二色胡枝子高效再生体系的优化,以二色胡枝子为材料,研究了6-BA、2,4-D、NAA等因素对其子叶节再生、节段扩繁和试管苗生根的影响。试验结果表明:子叶节再生最佳培养基为MS+6-BA0.1 mg/L;最佳增殖培养基为MS+6-BA0.1 mg/L+NAA0.01 mg/L;IBA,NAA对二色胡枝子试管苗的生根作用有影响,最佳生根培养基为1/2MS+IBA0.5 mg/L或者1/2MS+NAA0.1 mg/L。
In order to optimize the effective regeneration system of Lespedeza bicolor, the effects of 6- BA,2,4- D, NAA on cotyledonary node regeneration,proliferation and rooting of Lespedeza bicolor were studied. The results showed that the optimum regeneration medium was MS ＋ 6 - BA 0. 1mg,/L. MS ＋ 6 - BA 0.1 mg/L ＋ NAA 0.01 mg/L was an optimum proliferation medium. IBA and NAA played an important role in rooting and the optimum roofing medium was 1/2MS ＋ IBA 0.5 mg/L or 1/2MS ＋ NAA0.1mg/L.
出处
《河北林果研究》
2009年第2期117-120,共4页
Hebei Journal of Forestry and Orchard Research
基金
国家高技术研究发展计划(863计划)子项目"豆科饲料型灌木抗逆新品种选育"(2002AA241111)部分内容
关键词
二色胡枝子
再生体系优化
增殖
生根
Lespedeza bicolor
optimization of regeneration system
proliferation
rooting
