摘要
用乳酸钠作为丙酮酸的前体物质,生物合成L-苯基乙酰基甲醇(L-Phenylacetylcarbinol,L-PAC),控制NADH来抑制醇脱氢酶的活性降低副反应,提高酵母活细胞生物合成的L-PAC产量。实验结果表明,乳酸钠合成L-PAC的最佳反应条件为时间8 h,温度30℃,pH6.5,乳酸钠用量是22 mmol/L,L-PAC产量为12.3g/L。生产等量L-PAC所耗的丙酮酸为160 mmol/L,采用乳酸钠作为丙酮酸的替代品,可大幅度降低生产成本,并有利于工业化生产。
The yeast mediated preparation of L-phenylcetylcarbinol （L-PAC） usually utilizes fermenting yeast to condensed pyruvate with benzaldehyde. One of the main points of the reaction is the high cost of pyruvate. To solve it, sodium lactate was used as the precursor of pyruvate for this biosynthesis. Sodium lactate is oxidized with lactate dehydrogenase （LDH） in situ to pyruvate in yeast cells, which was then condensed with benzaldehyde. The results suggested that the yield reached the highest for eight hours＇ reaction at 30℃ and pH 6.5, with L-PAC yield of 12.3 g/L. Compared to the consumption of pyruvate 160mmol/L, which produces the equivalent yield of L-PAC, sodium lactate as the substitute can largely reduce the production cost in L-PAC industry.
出处
《药物生物技术》
CAS
CSCD
2009年第3期233-236,共4页
Pharmaceutical Biotechnology
