摘要
应用噻唑蓝法检测从赤魟中分离到的福安泰-03(Fuantai-03,FAT-03)对高转移人卵巢癌细胞HO-8910PM生长的影响;人工重组基底膜检测FAT-03对细胞侵袭能力的影响;SDS-聚丙烯酰胺凝胶电泳检测FAT-03对HO-8910PM细胞分泌MMP-9能力的影响。结果显示,FAT-03(20.0、40.0、60.0μg/ml)作用HO-8910PM细胞24h,对细胞生长的抑制率小于10%,对细胞与纤维粘连蛋白(fibronectin,FN)和Matrigel粘附的抑制率分别为15.1%、28.0%(P<0.05)、54.1%(P<0.01)和14.7%、34.3%(P<0.05)、40.5%(P<0.01),对细胞迁移的抑制率分别为16.4%、33.8%(P<0.05)和42.6%(P<0.01)。此外,FAT-03显著减少HO8910-PM细胞分泌MMP-9的量,并降低其活性。这些实验事实提示FAT-03能明显抑制HO-8910PM细胞的侵袭能力。
MTT assay was adapted to measure the effect of Fuantai-03 （FAT-03） isolated from Dasyatis akajei on the growth of human ovarian cancer HO-8910PM cells; artificial reconstituted basement membrane was utilized to evaluate the invasive activity; SDS-PAGE was employed to analyze the effects of FAT-03 on the activity and secretion of MMP-9 by HO-8910PM cells. The results showed that cell growth inhibition rates were 〈10% after the treatment of HO-8910PM with FAT-03 （20.0, 40.0, 60.0μg/ml） for 24 h, and that FAT-03 （20.0, 40.0, 60.0 μg/ ml） inhibited HO-8910PM cells to adhere to the basement membrane components FN and Matrigel by 15.1%, 28.0% （P〈0.05）, 54.1% （P〈0.01）, and 14.7%, 34.3% （P〈0.05）, 40.5% （P〈0.01）, respectively, and decreased the percentages of locomoting and migrating HO-8910PM cells by 16.4%, 33.8% （P〈0.05）, 42.6% （P〈0.01）, respectively. In addition, FAT-03 significantly inhibited the activity of MMP-9 and its secretion by HO-8910PM cells in a dosedependant manner. These experimental facts suggest that FAT-03 can significantly inhibit the invasion of HO- 8910PM cells.
出处
《细胞生物学杂志》
CAS
CSCD
2009年第3期384-388,共5页
Chinese Journal of Cell Biology
基金
国家自然科学基金(No.30271493)
广东省自然科学基金重点项目(No.021386)资助~~
