摘要
本文旨在研究慢性房颤患者左右心房胶原、基质金属蛋白酶(matrix metalloproteinases,MMPs)、基质金属蛋白酶抑制剂(tissue inhibitors of metalloproteinases,TIMPs)的mRNA水平是否存在差异。选择接受开胸换心脏瓣膜的手术病人共45例,其中慢性房颤患者27例,窦性心律患者18例;术中分别取左、右心耳组织,采用半定量RT-PCR法测定I型胶原、III型胶原、基质金属蛋白酶-1,9(MMP1、MMP9)以及基质金属蛋白酶抑制剂-1(TMP1)的mRNA表达水平。结果显示:(1)与窦性心律组相比,慢性房颤组患者左、右心房肌组织中I型胶原、MMP1、MMP9的mRNA表达水平均上调(P<0.05),TMP1的表达水平均下调(P<0.01);(2)窦性心律组患者左、右心房肌组之间I型胶原、III型胶原、MMP1、MMP9、TMP1的mRNA表达水平的差异均无统计学显著意义(P>0.05);(3)慢性房颤组患者MMP1的mRNA在右心房肌组织中表达水平较左心房高(P<0.05),MMP9的mRNA在左心房肌组织中表达水平较右心房高(P<0.01);(4)无论左心房还是右心房,MMP1、MMP9的mRNA表达水平均与I型胶原的mRNA表达水平呈正相关,与TMP1的mRNA表达水平呈负相关。以上结果表明,心房肌组织中MMP1/TMP1以及MMP9/TMP1的mRNA表达水平失衡引起I型胶原转录水平的改变,可能是慢性房颤患者心房肌间质纤维化的分子基础,在慢性房颤的过程中,MMPs的mRNA在左、右心房的表达是有差异的。
The present study aimed to investigate the mRNA expression levels of collagen, matrix metalloproteinases （MMPs）, and tissue inhibitors of metalloproteinases （TIMPs） in the left and right atria in patients with chronic atrial fibrillation （CAF）. Forty five patients with valvular heart disease were sampled in this study, including 18 patients with sinus rhythm （SR）, 27 patients with CAF.Clinical data of these patients were collected, and the left and right atrial appendages were obtained from these patients during heart valvular replacement surgery. The mRNA levels of collagen type I, collagen type III, MMP1, MMP9, TMP1 of the atria were then measured by semi-quantitative RT-PCR. The results obtained were as follows. Compared to those in SR group, in atria of CAF group,the mRNA levels of collagen type I, MMP1 and MMP9 increased （P〈0.05）, while the mRNA level of TMP1 decreased （P〈0.01）. There were no significant differences in the mRNA levels of collagen type I, collagen type III, MMP1, MMP9 and TMP1 between the left and right atria of SR group （P〉0.05）. In CAF group, the mRNA level of MMP1 in the right atrium was higher than that in the left atrium （P〈0.05）, however, the mRNA level of MMP9 in the left atrium was higher than that in the right atrium （P〈0.01）. In both the left and right atria, the mRNA of collagen type I was positively correlated with the corresponding atrial diameter; the mRNA of MMP1 and MMP9 was positively correlated with the mRNA of collagen type I, and was negatively correlated with the mRNA of TMP1.These results suggest that the increased level of collagen type I associated with selective upregulation of MMP 1, 9 and downregulation of TMP1, 9 in the atrium might be the molecular basis of atrial interstitial fibrosis in patients with CAF. Moreover, during CAF development, there is difference in the expression of MMPs between the left and right atria.
出处
《生理学报》
CAS
CSCD
北大核心
2009年第3期211-216,共6页
Acta Physiologica Sinica
关键词
心房颤动
胶原
基质金属蛋白酶
基质金属蛋白酶抑制剂
atrial fibrillation
collagen
matrix metalloproteinases
tissue inhibitors of metalloproteinases
