摘要
[目的]对2种猪肝基因组DNA提取方法,进行比较研究。[方法]分别采用试剂盒法和常规的氯仿/异戊醇抽提法,提取新鲜猪肝基因组DNA,并对其进行综合分析。[结果]试剂盒方法得到的DNA浓度较低,其OD260nm/OD280nm比值达到2.05,虽然蛋白质除的较干净,但是含RNA较高;而氯仿/异戊醇抽提法得到的DNA浓度高,其OD260nm/OD280nm比值1.88,纯度较高,且成本低。[结论]氯仿/异戊醇法对猪肝基因组DNA提取效果优于试剂盒法。
[Objective] The research aimed to compare the two extraction methods of genomic DNA from pork liver.[Method] kit method and chloroform/isoamyl alcohol extraction method were adopted to extract genomic DNA from fresh pork liver.[Result] The results showed that the DNA extraction kit could obtain high purity genomic DNA with low density, and high concentration, good quality and relatively low cost by chloroform/isoamyl alcohol method. [Conclusion] The chloroform/isoamyl alcohol extraction of genomic DNA is a more effective method of promoting and using in molecular studies on fresh pork liver.
出处
《安徽农业科学》
CAS
北大核心
2009年第18期8355-8356,共2页
Journal of Anhui Agricultural Sciences
基金
江西科技师范学院院级项目(2006)
江西省教育厅科技项目(赣教技字[2007]285号)
江西科技师范学院博士科研启动基金(2005)
关键词
猪肝
基因组DNA
氯仿/异戊醇抽提法
试剂盒法
比较
Pork liver
Genomic DNA
The chloroform/isoamyl alcohol extraction
kit method
Comparison
