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反定型A型试剂细胞中获得性类B抗原原因分析 被引量:1

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摘要 目的分析A型试剂红细胞出现获得性类B抗原的原因。方法使用血型血清学及基因分型技术鉴定混合A型试剂细胞中3个样本的ABO血型,确定有无未被检出的B亚型供者。用定型血清酸化试验、半乳糖胺中和试验,及3个不同批号的单克隆抗-B和人源抗-B定型试剂鉴定获得性类B抗原。结果送检A型试剂红细胞经证实为标准A型,未混有B型红细胞。发现A型试剂红细胞和正常B型红细胞在酸化后,以及半乳糖胺中和后与抗-B反应有显著差异,且被检A型试剂红细胞与不同批号的单克隆抗-B均不反应,而和人源抗-B定型试剂呈2+凝集反应。结论ABO反定型的误判因A型试剂红细胞存在获得性类B抗原引起,其可能因制作或贮存时污染所致。
机构地区 深圳市血液中心
出处 《中国输血杂志》 CAS CSCD 北大核心 2009年第5期391-393,共3页 Chinese Journal of Blood Transfusion
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