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庆大霉素产生菌GntB基因的克隆与转化

Cloning and Transformation of GntB Gene in Gentamicin-Producing Micromonospora
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摘要 根据小单孢菌产生庆大霉素的生物合成机理,利用基因克隆方法从棘孢小单孢菌(Micromonospora echinospora)基因组中扩增出庆大霉素生物合成的关键酶基因—2-脱氧青蟹肌糖合成酶基因(GntB),并将其通过大肠杆菌/链霉菌穿梭质粒pIJ699转化原菌株,采用硫链丝菌素抗性基因启动子带动2-脱氧青蟹肌糖合成酶基因在棘孢小单孢茵细胞中实现了转化。 On the basis of the biosynthesis mechanism of gentamicin-producing Micromonospora the key enzyme gene--2-deoxy-scyllo-inosose synthetase gene （GntB） of biosynthetic gentamicin was amplificated by gene-clone method and transformed to the original strain by shuttle plasmid pIJ699 of E. coli-Streptomyces and GntB was expressed in Micromonospora echinospora with thiostrepton resistant gene promoter driving GntB.
作者 管玉霞 刘志琼
机构地区 福州大学生物科学与工程学院
出处 《世界科技研究与发展》 CSCD 2009年第3期417-419,共3页 World Sci-Tech R&D
关键词 棘孢小单孢菌 2-脱氧青蟹肌糖合成酶基因(GntB) pIJ699 克隆 转化 Micromonospora echinospora 2-deoxy-scyllo-inosose synthetase gene （ GntB ） pIJ699 clone translation
分类号 Q785 [生物学—分子生物学]
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参考文献10

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世界科技研究与发展
2009年 第3期

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