摘要
目的:探讨毫针不同刺激强度针刺足三里穴后对胃扩张大鼠的拟痛行为学、脊髓背角N-甲基-D-天冬氨酸(NMDA)受体表达和P物质(SP)含量的影响.方法:50只大鼠按完全随机法分成5组,分别为空白组、假手术组、模型组、轻手法组和重手法组.采用胃内植入气囊并充气压40mmHg造成胃扩张疼痛模型,轻手法和重手法针刺足三里穴为施治因素,以拟痛行为学评分标准观察其拟痛行为学,用免疫组织化学法检测脊髓背角中NMDA受体表达和SP含量.结果:与假手术组比较,胃扩张造模后,大鼠拟痛行为学评分和脊髓背角内NMDA受体均明显增加(P<0.01);轻、重手法针刺均能明显降低胃扩张疼痛大鼠的疼痛反应,与针前比较有非常显著性意义(11.77±2.16分vs22.83±1.87分,14.73±2.19分vs21.73±2.30分,均P<0.01);并能降低NMDAR受体(140.60±21.30vs240.20±25.59,63.60±13.74vs108.67±11.37,均P<0.01)、SP阳性细胞数(63.60±13.74vs108.67±11.37,82.67±12.13vs108.67±11.37,均P<0.01),与模型组比较差异有非常显著性意义;且轻手法组优于重手法组(P<0.01).结论:不同刺激强度针刺足三里后,减轻了胃扩张疼痛大鼠的应激反应,其作用机制是抑制脊髓背角内疼痛相关信息物质NMDA受体激活和SP的释放.
AIM： To explore the effect of varied intensity acupuncture on pain-ethology scoring and on expression of n-methyl-d-aspartate receptor （NMDA receptor） and substance P （SP） in spinal dorsal horn quasi-pain in rats with pain induced by gastric distension. METHODS： Fifty healthy male Sprague Dawleyrats were randomly assigned to blank, sham operation, model, acupuncture of lighter stimulus intensity at ＂Zusanli＂ point and acupuncture of heavier stimulus intensity at ＂Zusanli＂ point groups. Gastric distention model was established by applying implanted aerostat in the gas- tric and filling in gas to 40 mmHg. Acupuncture of lighter and heavier stimulus intensity was applied. Pain-ethology scoring was observed and expression of NMDA receptor and SP was detected using immunohistochernical method. RESULTS： Compared with sham operation group, pain-ethology scoring and expression of NMDA receptor in spinal dorsal horn of model group were increased significantly （both P 〈 0.01）. After acupuncture, pain-ethology scoring was decreased significantly （11.77 ± 2.16 vs 22.83 ± 1.87, 14.73 ± 2.19 vs 21.73 ± 2.30, both P 〈 0.01）. Compared with model group, expression of NMDA receptor and SP in spinal dorsal horn of the two acupuncture groups was decreased significantly （140.60 ± 21.30 vs 240.20 ± 25.59, 63.60 ± 13.74 vs 108.67 ± 11.37; 63.60 ± 13.74 vs 108.67 ± 11.37, 82.67 ± 12.13 vs 108.67 ± 11.37, all P 〈 0.01）, and acupuncture of lighter stimulus intensity group had more obvious effect. CONCLUSION： Acupuncture at ＂Zusanli＂ could relieve stress reaction induced by gastric distension and decrease pain-ethology scoring, which is related to inhibited expression of pain correlation information substances （NMDA and SP）.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第13期1339-1345,共7页
World Chinese Journal of Digestology
基金
国家重大基础研究(973)基金资助项目
No.2005-CB523306-9
湖南省自然科学基金资助项目
No.06JJ50049
湖南省教育厅基金资助项目
No.08C632~~
关键词
毫针
刺激
胃扩张
疼痛
拟痛行为学评分
背根神经节
脊髓背角
N-甲基-D-天冬氨酸
P物质
Acupuncture
Stimulus
Gastric disten-tion
Visceral pain
Pain-ethology scoring
Dorsalroot ganglion
Comu posterus medullae spinalis
N-methyl-d-aspartate
Substance P
