摘要
分别用0(C)、0.75(T1)、1.50(T2)、2.25(T3)和3.0(T4)mg.头-1的抑制素pCIS基因疫苗免疫58头黄牛,以探讨抑制素pCIS基因免疫对卵泡发育和生殖激素的影响。结果表明,抑制素pCIS基因免疫组的大卵泡(d≥10 mm)数显著高于对照组(P<0.01),中卵泡(7 mm≤d<10 mm)数和小卵泡(4 mm≤d<7 mm)数与对照组均没有显著差异(P>0.05)。T1组左右两侧成熟卵泡大小与对照组差异不显著(P>0.05),其它3组两侧成熟卵泡均显著大于对照组(P<0.05)。抑制素pCIS基因免疫黄牛的促卵泡素(FSH)平均含量高于对照组,且在加强免疫阶段差异显著(P<0.05);加强免疫后雌二醇(E2)和孕酮(P4)含量均显著增加,且均与对照组差异显著(P<0.05)。这些结果表明,抑制素pCIS基因免疫可促进FSH分泌,进而影响黄牛卵泡发育和成熟。
To investigate the effect of inhibin gene immunization on follicle developments and reproductive hormone secretions,58 scalpers were immunized with 0 （Group C）,0. 75 （T1）, 1.50 （T2）, 2.25 （T3）, and 3.00 mg（T4） of inhibin eukaryotic expression plasmid （pCIS） respective- ly. The results showed that animals in groups T1, T2, T3 and T4 had a greater number of large follicles （≥10 mm） than those in group C, and had similar number of medium follicles （7 mm≤ d〈10 mm） or small （4 mm≤d〈7 mm） follicles（P〉0.05） to those in the group C. Moreover, follicles on bilateral ovaries of scalpers in inhibin gene vaccine immunized groups except for group T1 （P〉0.05）were significantly larger than those in group C （P〈0.05）. The levels of follicle stimulating hormone （FSH） in peripheral blood sera of scalpers immunized were higher than those of the control animals, and the difference was significant after a booster immunization（P〈 0.05）. Similarly, the levels of estradiol （E2） and progesterone （P4） in peripheral blood sera of scalpers immunized increased after a booster immunization, which were higher than those in the control animals（P〈0.05）. These results demonstrated that inhibin gene vaccine pCIS is able to stimulate FSH secretion, and to be beneficial to follicle development and maturation of scalpers.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第6期830-835,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金项目(30771549)
湖南农业大学人才稳定基金(2007WD03)
关键词
抑制素
基因免疫
卵泡发育
生殖激素
黄牛
inhibin
gene immunization
follicle development
reproductive hormone
scalper
