摘要
为调查我国鸡传染性法氏囊病病毒(IBDV)的流行情况,从我国11个省(市、自治区)采集法氏囊病料,分离鉴定鸡传染性法氏囊病病毒20株,运用RT-PCR方法对分离株的VP2基因进行扩增、测序和序列分析。研究表明,20株分离毒株中有19株具有IBDV超强毒株的分子特征,即222A、256I、294I和299S,与我国IBDV超强毒Gx株的核苷酸同源率在96.7%～99.4%,推导氨基酸同源率在98.2%～100%。遗传进化分析表明,所有分离毒株具有共同的祖先。
In this study,complete VP2 genes of 20 IBDV isolats from 11 provinces （city or antomous region）were amplified using RT-PCR and sequenced to determine IBDV molecular epidemiology. The results showed that a majority （19/20） of the isolates had very virulent IBDV （vvlBDV）conserved amino acids ：222A,2421,2561,294I and 299S,and only SH-h had P222. Compared to Chinese vvlBDV Gx strain,the homologous rates of nucleotide and amino atid were 97.5% to 99.4% and 98.9% to 100% ,respectively. Using serotype Ⅱ of IBDV as the outgroup, a phylogenetic tree based on VP2 nucleotide sequence was constructed. Our findings demonstrated that all isolates had a similar orgion with Gx strain and the other vvlBDV strains.
出处
《中国家禽》
北大核心
2009年第12期11-14,共4页
China Poultry
基金
国家肉鸡产业体系(nycytx-42-G3-01)
973基金资助项目(2005CB523202)
关键词
传染性法氏病病毒
分子流行病学
超强毒株
infectious bursal disease virus
molecular epidemiology
very viralent strain
