摘要
采用全国统一的9个鉴别寄主离体叶片法鉴定侵染型和Gilmour八进制法命名小种,对2004—2008年采自东北春麦区的辽宁、黑龙江等省不同县市的298个小麦白粉病菌标样进行生理小种及毒力频率分析,并利用已知抗白粉病基因的22个小麦品种(系)对参试菌株进行毒力频率测定。结果表明:2004—2006年优势小种为生理小种15号,出现频率分别为13.7%、15.6%和20.3%;2007年优势小种为17号小种,出现频率为10.2%;2008年411号小种上升为优势小种,频率达20.6%;2004—2008年毒性基因V4b、V2+6、V4+8、V12、V16、V18、V20、V21、V22和V23毒力频率较低,在0～28.0%之间,其对应抗性基因Pm4b、Pm2+6、Pm4+8、Pm12、Pm16、Pm18、Pm20、Pm21、Pm22和Pm23抗性较强,可作为有效的抗病基因加以利用。
In present paper, with a total of 298 isolates of Blumeria graminis f. sp. tritici collected in China＇ s northeastern spring wheat region during 2004 - 2008, the physiologic race and virulence types of the pathogen were investigated using detached leaf culture method of 9-nationwide unified differential hosts for infection type, Gilmour＇ s octal number system for race designation and 22 known Pm gene wheat varieties （lines） for virulence frequency analysis. The race survey result was indicated that race 15 maintained prevalent for 3 consecutive years： 2004 -2006 with the frequency of 13.7%, 15.6% and 20.3%, however, in the year 2007, the race 17 became most common with the frequency of 10.2%, furthermore, the prevailing race was shifted into race 411 with the frequency of 20.6% in the year 2008. The virulence frequency result for 2004 -2008 was shown that the virulent genes V4b, V2 ＋ 6, 114 ＋ 8, V12, V16, V18, V20, V21, V22, V23 occurred in very low amount, their virulence frequency being 0 - 28.0%, in other words, Pm4b, Pm2 ＋6, Pm4 ＋8, Pm12, Pm16, Pm18, Pm20, Pm21, Pm22, Pm23 were effective and had applicable value in wheat breeding for resistance.
出处
《植物保护学报》
CAS
CSCD
北大核心
2009年第3期213-218,共6页
Journal of Plant Protection
基金
国家科技攻关(2004BA509B03)
国家科技支撑计划(2006BAD08A05)
公益性行业(农业)科研专项(3-15)
关键词
小麦白粉病菌
生理小种
毒力频率
抗白粉病基因
Blumeria graminis f. sp. tritici
physiological race
virulence frequency
Pm genes
