摘要
本实验主要对榆耳菌菌丝体多糖提取工艺进行研究,以提高产品的得率。通过单因素试验及正交试验,得纤维素酶提取榆耳菌丝体胞内多糖的最佳工艺条件:料水比1:60、pH5.3、温度43℃、酶解时间2.0h、加酶量2.5%,在此条件下,榆耳菌丝体胞子内多糖的提取率为4.86%。
Cellulase-catalyzed hydrolysis was used to extract polysaccharides from the collected mycelia of Gloeostereum incarnatum 105 after submerged fermentation. The extraction technology was optimized by orthogonal factorial design based on single factor test. The results showed that the optimal conditions for extracting polysaccharides from mycelia of Gloeostereum incarnatum 105 were as follows： solid/liquid ratio 1：60, pH 5.3, cellulose amount 2,5%, and hydrolysis temperature 43 ℃ for a hydrolysis duration of 2.0 h; under these conditions, the yield of polysaccharides reached 4.86%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第13期201-204,共4页
Food Science
基金
吉林省教育厅资助项目(吉教科合字2006055)
关键词
榆耳
多糖
酶解
纤维素酶
Gloeostereum insarnatum
polysaccharides
enzymatic hydrolysis
cellulase
