摘要
目的探讨磁共振对比剂超小超顺磁性氧化铁粒子(USPIO)增强磁共振(MR)检出实验性变态反应性脑脊髓炎(EAE)动物模型病变的敏感性及其增强病灶的磁化传递率(MTR)的变化。方法11只EAE组大鼠及10只对照组大鼠行常规T1加权像、T2加权像、三维T1磁化传递、三维T1非磁化传递、钆对比剂(钆喷酸葡胺注射液)增强T1加权像及USPIO增强T2加权像扫描。计算USPIO及钆增强检出EAE病变的敏感性、EAE组大鼠第1次出现US-PIO异常强化的区域及前一次扫描相应区域的MTR。大鼠脑组织行HE及髓鞘染色。结果钆对比剂增强扫描未发现EAE大鼠脑内异常对比增强灶,USPIO增强扫描显示11只大鼠脑内均有异常沉积。EAE组大鼠USPIO异常强化区的MTR值与出现强化前同一区域的MTR值相比差异具有显著性(P<0.05)。USPIO强化区可见炎细胞浸润及脱髓鞘改变,对照组大鼠MR平扫及增强扫描无阳性显示。结论USPIO增强MR检出EAE病变的敏感性高。与USPIO增强扫描相结合,磁化传递成像能够提示EAE病变的性质。
Objective To explore the sensitivity of magnetic resonance contrast agent ultrasmall superparamagnetic iron oxide （USPIO） enhancement scan in detecting experimental allergic encephalopathy （EAE） lesions and the change of magnetic transfer of USPIO enhancement lesions in the animal model of EAE. Methods The routine Tl-weighted imaging, T2-weighted imaging, three dimensional T1 magnetic transfer, three dimensional T1 no magnetic transfer, Dimeglumine Gadopentetate injection （Gd-DTPA） enhancement, and USPIO enhancement scan were performed in 11 EAE rats and 10 control rats respectively. The sensitivity of USPIO and Gd-DTPA enhancement in detecting the lesions in EAE rats was calculated. Magnetic transfer ratio （MTR） of USPIO enhancement area for the first time in EAE rats and MTR of the same area of the last scan were calculated respectively. HE and myelin staining of brain tissues were performed. Results No abnormally enhanced lesions were showed in EAE rats＇ brain in Gd-DTPA enhancement scan, whiIe abnormally enhanced lesions were showed in 11 EAE rats＇ brain in USPIO enhancement scan. The MTR value of USPIO enhancement area for the first time was significantly different from MTR of the same area of the last scan in EAE rats （P 〈 0.05 ）. Inflammation cells and demyelination lesions were found in USPIO enhancement area histopathologically. There were no positive findings in control rats. Conclusions The sensitivity of USPIO en- hancement scan in detecting EAE lesions was high. Magnetic transfer imaging, together with USPIO enhancement scan, was helpful to determine the features of the EAE lesions.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2009年第2期177-181,I0005,共6页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金(30600578)
北京市科技新星计划(2007A102)~~
关键词
磁共振
对比剂
多发性硬化
实验性变态反应性脑脊髓炎
magnetic resonance
contrast agent
multiple sclerosis
experimental allergic encephalopathy
