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Reactive Oxygen Species Scavenging Enzymes and Down-Adjustment of Metabolism Level in Mitochondria Associated with Desiccation-Tolerance Acquisition of Maize Embryo 被引量:2

Reactive Oxygen Species Scavenging Enzymes and Down-Adjustment of Metabolism Level in Mitochondria Associated with Desiccation-Tolerance Acquisition of Maize Embryo
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摘要 It is a well-known fact that a mature seed can survive losing most of its water, yet how seeds acquire desiccation- tolerance is not well understood. Through sampling maize embryos of different developmental stages and comparatively studying the integrity, oxygen consumption rate and activities of antioxidant enzymes in the mitochondria, the main origin site of reactive oxygen species (ROS) production in seed cells, we found that before an embryo achieves desiccation-tolerance, its mitochondria shows a more active metabolism, and might produce more ROS and therefore need a more effective ROS scavenging system. However, embryo dehydration in this developmental stage declined the activities of most main antioxidant enzymes and accumulated thiobarbituric acid-reactive products in mitochondria, and then destroyed the structure and functional integrity of mitochondria. In physiologically-matured embryos (dehydration- tolerant), mitochondria showed lower metabolism levels, and no decline in ROS scavenging enzyme activities and less accumulation of thiobarbituric acid-reactive products after embryo dehydration. These data indicate that seed desiccation- tolerance acquisition might be associated with down-adjustment of the metabolism level in the late development stage, resulting in less ROS production, and ROS scavenging enzymes becoming desiccation-tolerant and then ensuring the structure and functional integrity of mitochondria. It is a well-known fact that a mature seed can survive losing most of its water, yet how seeds acquire desiccation- tolerance is not well understood. Through sampling maize embryos of different developmental stages and comparatively studying the integrity, oxygen consumption rate and activities of antioxidant enzymes in the mitochondria, the main origin site of reactive oxygen species (ROS) production in seed cells, we found that before an embryo achieves desiccation-tolerance, its mitochondria shows a more active metabolism, and might produce more ROS and therefore need a more effective ROS scavenging system. However, embryo dehydration in this developmental stage declined the activities of most main antioxidant enzymes and accumulated thiobarbituric acid-reactive products in mitochondria, and then destroyed the structure and functional integrity of mitochondria. In physiologically-matured embryos (dehydration- tolerant), mitochondria showed lower metabolism levels, and no decline in ROS scavenging enzyme activities and less accumulation of thiobarbituric acid-reactive products after embryo dehydration. These data indicate that seed desiccation- tolerance acquisition might be associated with down-adjustment of the metabolism level in the late development stage, resulting in less ROS production, and ROS scavenging enzymes becoming desiccation-tolerant and then ensuring the structure and functional integrity of mitochondria.
出处 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2009年第7期638-645,共8页 植物学报(英文版)
基金 Supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-YW-Z-058) the National Natural Science Foundation of China (30470183 30870223)
关键词 DESICCATION-TOLERANCE developing embryo MITOCHONDRIA reactive oxygen species scavenging enzymes desiccation-tolerance developing embryo mitochondria reactive oxygen species scavenging enzymes
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  • 1Arrigoni O, Gara LD, Tommasi F, Liso R (1992). Changes in the ascorbate system during seed development of Vicia faba L. Plant Physiol. 99, 235-238.
  • 2Bailly C, Audigier C, Ladonne F, Wagner MH, Coste F, Corbineau F et al. (2001). Changes in oligosaccharide content and antioxidant enzyme activities in developing bean seeds is related to acquisition of drying tolerance and seed quality. J. Exp. Bot. 52, 701-708.
  • 3Bartels D, Singh M, Salamini F (1988). Onset of desiccation tolerance during development of the barley embryo. Planta 175, 485-492.
  • 4Bartoli CG, Gomez F, Martinez DE, Guiamet JJ (2004). Mitochondria are the main target for oxidative damage in leaves of wheat (Triticum aestivum L.). J. Exp. Bot. 55, 1663-1669.
  • 5Benamar A, Tallon C, Macherel D (2003). Membrane integrity and oxidative properties of mitochondria isolated from imbibing pea seeds after priming or accelerated ageing. Seed Sci. Res. 13, 35-45.
  • 6Berjak P (2006). Unifying perspectives of some mechanisms basic to desiccation tolerance across life forms. Seed Sci. Res. 16, 1-15.
  • 7Bernacchiaa G, Furini A (2004). Biochemical and molecular responses to water stress in resurrection plants. Physiol. Plant. 121, 175-181.
  • 8Bewley JB, Black M (1994). Seeds: Physiology of Development and Germination. Plenum Press, New York. pp. 117-145.
  • 9Beyer W, Fridovich I (1987). Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal. Biochem. 161,559-566.
  • 10Black M, Corbineau F, Grzesik M, Guy P, Come D (1996). Carbohydrate metabolism in the developing and maturing wheat embryo in relation to its desiccation tolerance. J. Exp. Bot. 47, 161-169.

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