摘要
以不结球白菜子叶型小孢子胚为外植体,研究冷处理、活性炭及AgNO3对小孢子植株再生的影响,并对再生植株染色体倍性进行鉴定.结果表明:对小孢子胚进行5d的4℃冷处理培养能提高其胚芽诱导率和胚芽数;培养基中添加1.0g/L的活性炭对提高小孢子胚芽诱导率没有明显效果,但能有效减轻胚芽的玻璃化;添加5.0或7.0mg/L的AgNO3对小孢子胚芽诱导有显著效果.染色体倍性鉴定结果表明:不结球白菜小孢子植株的染色体自然加倍率较高,在50%～100%之间;不同基因型不结球白菜小孢子植株的倍性变异具有多样性;在部分基因型中嵌合体占较高比例,最高达到42.86%.
Microspore-derived cotyledonary embryos of non-heading Chinese cabbage were used as explants. Effects of chilling,activated charcoal and AgN03 on plant regeneration were studied,and the chromosome ploidy of regenerated plants was identified. The results showed that cold treatment（4℃） for 5d increased the rate of embryo germination and the number of shoots. Adding activated charcoal（1.0 g/L） in the medium showed no distinct difference for embryo germination,but the phenomenon of vitrification had been relieved. Adding 5.0 or 7.0 mg/L AgNO3 in the medium efficiently improved the embryo germination. The ploidy analysis results showed that non-heading Chinese cabbage had a high level（50%-100%） of spontaneous chromosome doubling rate,the ploidy variation of different genotypes had diversity. The rate of chimera was high in some genotypes with the highest value of 42.86 %.
出处
《西北植物学报》
CAS
CSCD
北大核心
2009年第6期1091-1096,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
教育部科技重点项目(106092)
新世纪优秀人才支持计划(NCET-07-0439)
关键词
不结球白菜
小孢子胚
植株再生
倍性
non-heading Chinese cabbage
microspore-derived embryos
plant development
ploidy
