摘要
目的研究血清铜络合蛋白（ＣＣＰ）在癌症患者的改变。方法人血清经Ｃｕ２＋处理后，通过１４％聚丙烯酰胺凝胶（ＰＡＧＥ，交联度１％）垂直平板电泳技术检测ＣＣＰ蛋白质区带的变化。结果经Ｃｕ２＋处理后的血清较原血清电泳区带新增一条区带，即ＣＣＰ带；发现在３３８例癌症患者电泳区带中ＣＣＰ（％）为２３±０８，显著低于１４６例正常人（５８±１４）和９５例非癌症患者（５４±１６，Ｐ＜００１），尤其在恶性淋巴瘤患者减低更为显著，与正常组和非癌症患者组差异无显著意义。实验发现ＣＣＰ的产生可能与富含巯基的白蛋白有关。
Objective To study the changes of copper complex protein (CCP) in cancer patients.Methods After human serum was treated with ionic copper, protein zones were separated by 14% PAGE (crosslinkage rate 1%) vertical slab electrophoresis.Results A new zone was detected in the treated serum with ionic copper. In the CCP zone of 338 cancer patients, 146 normal controls, and 95 noncancer patients the percentage of CCP was 2.3±0.8, 5.8±14, 5.4±1.6, respectively. Cancer patients showed significantly reduced CCP values (P<0.01), especially in those with malignant lymphoma. There was no significant difference between the percentage of CCP in normal controls and noncancer patients. The origin of CCP was possibly related to albumin binding mercaptoethanesulphonic acid (mesna) in experiment.Conclusions CCP is of value in the diagnosis and treatment of cancer patients.
